LIMMS/CNRS-IIS, UMI 2820, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8505, Japan.
Drug Discov Today. 2013 Jun;18(11-12):552-9. doi: 10.1016/j.drudis.2013.02.002. Epub 2013 Feb 9.
To progress in basic science and drug development, convenient methodology for detecting specific biological molecules and their interaction in living organism is in high demand. After more than 20 years of increasing research efforts, micro and nanotechnologies are now mature to propose a new class of miniature devices and principles enabling compartmentalized bioassays. Among them, this review proposes various examples that include array of electro-active microwells for highly parallel single cell analysis, cost-effective nanofluidic for DNA separation, parallel enzymatic reaction in 100pL droplet and high-throughput platform for membrane proteins assays. The micro devices are presented with relevant experiments to foresee their future contribution to translational research and drug discovery.
为了在基础科学和药物开发方面取得进展，人们迫切需要一种方便的方法来检测生物体内特定的生物分子及其相互作用。经过 20 多年来不断增加的研究努力，微纳技术现在已经成熟，可以提出一类新的微型设备和原理，实现分隔式生物测定。在这些技术中，本综述提出了各种示例，包括用于高通量单细胞分析的电活性微井阵列、具有成本效益的纳流道用于 DNA 分离、在 100pL 液滴中进行平行酶反应以及用于膜蛋白测定的高通量平台。本文还介绍了相关实验，以预见它们对转化研究和药物发现的未来贡献。
