Shoemaker R L, Yamazaki R K
Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
Biochim Biophys Acta. 1990 May 1;1044(1):169-72. doi: 10.1016/0005-2760(90)90233-n.
In studies using primary cultures of adult rat hepatocytes in serum-free medium, peroxisomal fatty acyl-CoA oxidase activity was not altered by the presence of 3,5,3'-triiodothyronine, whereas time- and dose-dependent increases in the thyroid hormone-responsive enzyme mitochondrial glycero-3-phosphate dehydrogenase were seen. Activity of peroxisomal oxidase was stimulated with clofibric acid in the absence of 3,5,3'-triiodothyronine. The results demonstrate that hepatic peroxisomal fatty acyl-CoA oxidase activity is not directly regulated by 3,5,3'-triiodothyronine and that stimulation of peroxisomal fatty acyl-CoA oxidase activity by clofibric acid does not require thyroid hormone.
在使用无血清培养基中成年大鼠原代肝细胞进行的研究中，3,5,3'-三碘甲状腺原氨酸的存在并未改变过氧化物酶体脂肪酰辅酶A氧化酶的活性，而甲状腺激素反应性酶线粒体甘油-3-磷酸脱氢酶则出现了时间和剂量依赖性增加。在不存在3,5,3'-三碘甲状腺原氨酸的情况下，氯贝酸刺激了过氧化物酶体氧化酶的活性。结果表明，肝脏过氧化物酶体脂肪酰辅酶A氧化酶的活性不受3,5,3'-三碘甲状腺原氨酸的直接调节，并且氯贝酸对过氧化物酶体脂肪酰辅酶A氧化酶活性的刺激不需要甲状腺激素。
