Chemical Engineering Department, ETSEIAT, Universitat Politècnica de Catalunya, Colom 11, E-08222 Terrassa, Spain.
Bioresour Technol. 2013 Mar;131:536-40. doi: 10.1016/j.biortech.2013.01.033. Epub 2013 Jan 22.
In this work, kenaf pulp was delignified by using laccase in combination with various redox mediators and the efficiency of the different laccase–mediator systems assessed in terms of the changes in pulp properties after bleaching. The oxidative ability of the individual mediators used (acetosyringone, syringaldehyde, p-coumaric acid, vanillin and actovanillone) and the laccase–mediator systems was determined by monitoring the oxidation–reduction potential (ORP) during process. The results confirmed the production of phenoxy radicals of variable reactivity and stressed the significant role of lignin structure in the enzymatic process. Although changes in ORP were correlated with the oxidative ability of the mediators, pulp properties as determined after the bleaching stage were also influenced by condensation and grafting reactions. As shown here, ORP measurements provide a first estimation of the delignification efficiency of a laccase–mediator system.
在这项工作中，使用漆酶结合各种氧化还原介体对剑麻浆进行脱木质素处理，并根据漂白后纸浆性能的变化来评估不同漆酶-介体系统的效率。通过监测过程中的氧化还原电位 (ORP)，确定了所用的各个介体（乙酰丁香酮、丁香醛、对香豆酸、香草醛和香草乙酮）和漆酶-介体系统的氧化能力。结果证实了具有不同反应性的酚氧基自由基的产生，并强调了木质素结构在酶促过程中的重要作用。尽管 ORP 的变化与介体的氧化能力相关，但漂白阶段后确定的纸浆性能也受到缩合和接枝反应的影响。如本文所示，ORP 测量值可初步估算漆酶-介体系统的脱木质素效率。
