Key Laboratory for Medical Tissue Regeneration of Henan Province, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China.
Auton Neurosci. 2013 Jun;176(1-2):48-53. doi: 10.1016/j.autneu.2013.01.007. Epub 2013 Feb 9.
The dorsal motor nucleus of vagus (DMV) plays an important role in the regulation of gastrointestinal function. Dopamine (DA) exerts potent neuromodulatory effects on the motoneurons in the DMV via dopamine receptors (DRs). However, the distribution of DRs and their neurochemical phenotypes in the DMV are unclear. In the present study, the distribution of DRs D1- and D2-immunoreactive (IR) neurons and their neurochemical phenotypes in the DMV of rats were investigated using a double-labeling immunofluorescence technique combined with confocal microscopy. The results indicated that a considerable quantity of D1 and D2 was expressed throughout the DMV. A large amount of choline acetyltransferase (ChAT)-IR and a few tyrosine hydroxylase (TH)-IR neurons were observed in the DMV. Nearly all of the neurons were also D1-IR and D2-IR. In conclusion, the present study demonstrates the wide distribution of D1 and D2 in the cholinergic and catecholaminergic neurons in the DMV of rats. The DRs might play an important role in the regulation of DA on the activity of cholinergic and catecholaminergic neurons in the DMV.
迷走神经背核（DMV）在胃肠功能调节中起着重要作用。多巴胺（DA）通过多巴胺受体（DRs）对 DMV 中的运动神经元发挥强大的神经调制作用。然而，DRs 在 DMV 中的分布及其神经化学表型尚不清楚。在本研究中，采用双重免疫荧光技术结合共聚焦显微镜，研究了大鼠 DMV 中 DRs D1-和 D2-免疫反应性（IR）神经元及其神经化学表型的分布。结果表明，大量 D1 和 D2 在 DMV 中表达。在 DMV 中观察到大量的胆碱乙酰转移酶（ChAT）-IR 和少量的酪氨酸羟化酶（TH）-IR 神经元。几乎所有的神经元也都是 D1-IR 和 D2-IR。总之，本研究表明 D1 和 D2 在大鼠 DMV 中的胆碱能和儿茶酚胺能神经元中广泛分布。DRs 可能在调节 DA 对 DMV 中胆碱能和儿茶酚胺能神经元活性的作用中发挥重要作用。
