• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

甲型流感病毒非结构蛋白 1 与人鸟苷酸结合蛋白 1 相互作用拮抗抗病毒活性。

Nonstructural protein 1 of influenza A virus interacts with human guanylate-binding protein 1 to antagonize antiviral activity.

机构信息

Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.

出版信息

PLoS One. 2013;8(2):e55920. doi: 10.1371/journal.pone.0055920. Epub 2013 Feb 6.

DOI:10.1371/journal.pone.0055920
PMID:23405236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3566120/
Abstract

Human guanylate-binding protein 1 (hGBP1) is an interferon-inducible protein involved in the host immune response against viral infection. In response to infection by influenza A virus (IAV), hGBP1 transcript and protein were significantly upregulated. Overexpression of hGBP1 inhibited IAV replication in a dose-dependent manner in vitro. The lysine residue at position 51 (K51) of hGBP1 was essential for inhibition of IAV replication. Mutation of K51 resulted in an hGBP1 that was unable to inhibit IAV replication. The viral nonstructural protein 1 (NS1) was found to interact directly with hGBP1. K51 of hGBP1 and a region between residues 123 and 144 in NS1 were demonstrated to be essential for the interaction between NS1 and hGBP1. Binding of NS1 to hGBP1 resulted in a significant reduction in both GTPase activity and the anti-IAV activity of hGBP1. These findings indicated that hGBP1 contributed to the host immune response against IAV replication and that hGBP1-mediated antiviral activity was antagonized by NS1 via binding to hGBP1.

摘要

人鸟苷酸结合蛋白 1（hGBP1）是一种干扰素诱导的蛋白，参与宿主对病毒感染的免疫反应。在甲型流感病毒（IAV）感染时，hGBP1 的转录本和蛋白水平显著上调。体外实验中，hGBP1 的过表达以剂量依赖性方式抑制 IAV 复制。hGBP1 第 51 位赖氨酸残基（K51）对于抑制 IAV 复制是必需的。K51 突变导致 hGBP1 无法抑制 IAV 复制。发现病毒非结构蛋白 1（NS1）与 hGBP1 直接相互作用。hGBP1 的 K51 残基和 NS1 中 123 到 144 位残基之间的区域对于 NS1 和 hGBP1 之间的相互作用是必需的。NS1 与 hGBP1 的结合导致 hGBP1 的 GTPase 活性和抗 IAV 活性显著降低。这些发现表明，hGBP1 有助于宿主对 IAV 复制的免疫反应，并且 NS1 通过与 hGBP1 结合拮抗 hGBP1 介导的抗病毒活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/56cc794031d6/pone.0055920.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/d88208018ef0/pone.0055920.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/ebac5b8a0103/pone.0055920.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/8b3ae6b3007e/pone.0055920.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/abcfc3c52eed/pone.0055920.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/97776533cafd/pone.0055920.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/c11b2e374c83/pone.0055920.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/fad60e2a03ac/pone.0055920.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/56cc794031d6/pone.0055920.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/d88208018ef0/pone.0055920.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/ebac5b8a0103/pone.0055920.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/8b3ae6b3007e/pone.0055920.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/abcfc3c52eed/pone.0055920.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/97776533cafd/pone.0055920.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/c11b2e374c83/pone.0055920.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/fad60e2a03ac/pone.0055920.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be29/3566120/56cc794031d6/pone.0055920.g008.jpg

相似文献

1
Nonstructural protein 1 of influenza A virus interacts with human guanylate-binding protein 1 to antagonize antiviral activity.甲型流感病毒非结构蛋白 1 与人鸟苷酸结合蛋白 1 相互作用拮抗抗病毒活性。
PLoS One. 2013;8(2):e55920. doi: 10.1371/journal.pone.0055920. Epub 2013 Feb 6.
2
Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor.甲型流感病毒非结构蛋白 NS1 和 NS2 在自然细胞感染过程中的定量蛋白质组学分析鉴定 PACT 为 NS1 靶蛋白和抗病毒宿主因子。
J Virol. 2014 Aug;88(16):9038-48. doi: 10.1128/JVI.00830-14. Epub 2014 Jun 4.
3
Activation of c-jun N-terminal kinase upon influenza A virus (IAV) infection is independent of pathogen-related receptors but dependent on amino acid sequence variations of IAV NS1.流感病毒（IAV）感染后 c-jun N 末端激酶的激活不依赖于病原体相关受体，但依赖于 IAV NS1 的氨基酸序列变异。
J Virol. 2014 Aug;88(16):8843-52. doi: 10.1128/JVI.00424-14. Epub 2014 May 28.
4
The Short Form of the Zinc Finger Antiviral Protein Inhibits Influenza A Virus Protein Expression and Is Antagonized by the Virus-Encoded NS1.锌指抗病毒蛋白的短形式抑制甲型流感病毒蛋白表达，并被病毒编码的NS1拮抗。
J Virol. 2017 Jan 3;91(2). doi: 10.1128/JVI.01909-16. Print 2017 Jan 15.
5
Epigenetic Modification Is Regulated by the Interaction of Influenza A Virus Nonstructural Protein 1 with the DNA Methyltransferase DNMT3B and Subsequent Transport to the Cytoplasm for K48-Linked Polyubiquitination.表观遗传修饰受流感 A 病毒非结构蛋白 1 与 DNA 甲基转移酶 DNMT3B 的相互作用调控，并随后转运至细胞质进行 K48 连接的多泛素化。
J Virol. 2019 Mar 21;93(7). doi: 10.1128/JVI.01587-18. Print 2019 Apr 1.
6
Influenza a virus NS1 protein induced A20 contributes to viral replication by suppressing interferon-induced antiviral response.甲型流感病毒NS1蛋白诱导的A20通过抑制干扰素诱导的抗病毒反应促进病毒复制。
Biochem Biophys Res Commun. 2017 Jan 22;482(4):1107-1113. doi: 10.1016/j.bbrc.2016.11.166. Epub 2016 Dec 1.
7
Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells.甲型流感病毒 NS1 效应结构域是感染细胞中 PA-X 介导的宿主关闭所必需的。
J Virol. 2024 May 14;98(5):e0190123. doi: 10.1128/jvi.01901-23. Epub 2024 Apr 17.
8
A Conserved Residue, Tyrosine (Y) 84, in H5N1 Influenza A Virus NS1 Regulates IFN Signaling Responses to Enhance Viral Infection.H5N1 流感 A 病毒 NS1 中一个保守的残基酪氨酸（Y）84 调节 IFN 信号反应以增强病毒感染。
Viruses. 2017 May 12;9(5):107. doi: 10.3390/v9050107.
9
A Novel Role for PDZ-Binding Motif of Influenza A Virus Nonstructural Protein 1 in Regulation of Host Susceptibility to Postinfluenza Bacterial Superinfections.甲型流感病毒非结构蛋白 1 的 PDZ 结合基序在调控宿主对流感后细菌合并感染易感性中的新作用。
Viral Immunol. 2019 Apr;32(3):131-143. doi: 10.1089/vim.2018.0118. Epub 2019 Mar 1.
10
Antiviral activity of double-stranded RNA-binding protein PACT against influenza A virus mediated via suppression of viral RNA polymerase.双链 RNA 结合蛋白 PACT 通过抑制流感病毒 RNA 聚合酶发挥抗病毒活性。
FASEB J. 2018 Aug;32(8):4380-4393. doi: 10.1096/fj.201701361R. Epub 2018 Mar 7.

引用本文的文献

1
The Junction Between nsp1β and nsp2 in the Porcine Reproductive and Respiratory Syndrome Virus Genome Is a New Site for the Insertion and Expression of Foreign Genes.猪繁殖与呼吸综合征病毒基因组中nsp1β与nsp2之间的连接区是外源基因插入和表达的新位点。
Viruses. 2025 Apr 30;17(5):656. doi: 10.3390/v17050656.
2
Antiviral roles of eosinophils in asthma and respiratory viral infection.嗜酸性粒细胞在哮喘和呼吸道病毒感染中的抗病毒作用。
Front Allergy. 2025 Feb 28;6:1548338. doi: 10.3389/falgy.2025.1548338. eCollection 2025.
3
NS2B-D55E and NS2B-E65D Variations Are Responsible for Differences in NS2B-NS3 Protease Activities Between Japanese Encephalitis Virus Genotype I and III in Fluorogenic Peptide Model.

本文引用的文献

1
Stabilization of p53 in influenza A virus-infected cells is associated with compromised MDM2-mediated ubiquitination of p53.甲型流感病毒感染细胞中 p53 的稳定与 MDM2 介导的 p53 泛素化作用受损有关。
J Biol Chem. 2012 May 25;287(22):18366-75. doi: 10.1074/jbc.M111.335422. Epub 2012 Apr 3.
2
A new splice variant of the human guanylate-binding protein 3 mediates anti-influenza activity through inhibition of viral transcription and replication.一种新型人类鸟苷酸结合蛋白 3 的剪接变体通过抑制病毒转录和复制来介导抗流感活性。
FASEB J. 2012 Mar;26(3):1290-300. doi: 10.1096/fj.11-189886. Epub 2011 Nov 21.
3
Determinants of GBP recruitment to Toxoplasma gondii vacuoles and the parasitic factors that control it.
NS2B-D55E和NS2B-E65D变异导致了荧光肽模型中日本脑炎病毒I型和III型之间NS2B-NS3蛋白酶活性的差异。
Int J Mol Sci. 2024 Nov 26;25(23):12680. doi: 10.3390/ijms252312680.
4
Mouse guanylate-binding proteins of the chromosome 3 cluster do not mediate antiviral activity in vitro or in mouse models of infection.鼠染色体 3 簇鸟苷酸结合蛋白在体外或在感染的小鼠模型中不介导抗病毒活性。
Commun Biol. 2024 Aug 25;7(1):1050. doi: 10.1038/s42003-024-06748-8.
5
Host Innate Antiviral Response to Influenza A Virus Infection: From Viral Sensing to Antagonism and Escape.宿主对甲型流感病毒感染的先天性抗病毒反应：从病毒感知到拮抗作用与逃逸
Pathogens. 2024 Jul 3;13(7):561. doi: 10.3390/pathogens13070561.
6
The pseudogene GBP1P1 suppresses influenza A virus replication by acting as a protein decoy for DHX9.GBP1P1 假基因通过充当 DHX9 的蛋白诱饵来抑制甲型流感病毒复制。
J Virol. 2024 Jul 23;98(7):e0073824. doi: 10.1128/jvi.00738-24. Epub 2024 Jun 28.
7
Immunogenicity and Protective Capacity of Sugar ABC Transporter Substrate-Binding Protein against Serotype 2, 7 and 9 Infection in Mice.糖ABC转运体底物结合蛋白对小鼠2型、7型和9型感染的免疫原性及保护能力
Vaccines (Basel). 2024 May 15;12(5):544. doi: 10.3390/vaccines12050544.
8
Development of Colloidal Gold-Based Immunochromatographic Strips for Rapid Detection and Surveillance of Japanese Encephalitis Virus in Dogs across Shanghai, China.胶体金免疫层析条的研制及其在中国上海地区犬体内日本脑炎病毒的快速检测和监测。
Viruses. 2024 Feb 6;16(2):258. doi: 10.3390/v16020258.
9
Influenza Virus Host Restriction Factors: The ISGs and Non-ISGs.流感病毒宿主限制因子：干扰素刺激基因和非干扰素刺激基因
Pathogens. 2024 Jan 29;13(2):127. doi: 10.3390/pathogens13020127.
10
Guanylate-Binding Protein 2 Exerts GTPase-Dependent Anti-Ectromelia Virus Effect.鸟苷酸结合蛋白2发挥依赖于GTP酶的抗埃可病毒作用。
Microorganisms. 2023 Sep 8;11(9):2258. doi: 10.3390/microorganisms11092258.
弓形虫 vacuoles 中 GBP 的募集决定因素及其调控的寄生虫因子。
PLoS One. 2011;6(9):e24434. doi: 10.1371/journal.pone.0024434. Epub 2011 Sep 8.
4
Transcription analysis on response of swine lung to H1N1 swine influenza virus.猪肺对 H1N1 猪流感病毒反应的转录分析。
BMC Genomics. 2011 Aug 8;12:398. doi: 10.1186/1471-2164-12-398.
5
A family of IFN-γ-inducible 65-kD GTPases protects against bacterial infection.IFN-γ 诱导的 65kD GTPases 家族可预防细菌感染。
Science. 2011 May 6;332(6030):717-21. doi: 10.1126/science.1201711.
6
A diverse range of gene products are effectors of the type I interferon antiviral response.多种基因产物是 I 型干扰素抗病毒反应的效应物。
Nature. 2011 Apr 28;472(7344):481-5. doi: 10.1038/nature09907. Epub 2011 Apr 10.
7
The guanylate-binding proteins: emerging insights into the biochemical properties and functions of this family of large interferon-induced guanosine triphosphatase.鸟苷酸结合蛋白：对该家族大干扰素诱导鸟苷三磷酸酶的生化特性和功能的新认识。
J Interferon Cytokine Res. 2011 Jan;31(1):89-97. doi: 10.1089/jir.2010.0102. Epub 2010 Dec 13.
8
Antiviral effects of the interferon-induced protein guanylate binding protein 1 and its interaction with the hepatitis C virus NS5B protein.干扰素诱导蛋白鸟苷酸结合蛋白1的抗病毒作用及其与丙型肝炎病毒NS5B蛋白的相互作用
Hepatology. 2009 Dec;50(6):1727-37. doi: 10.1002/hep.23195.
9
Human guanylate binding proteins potentiate the anti-chlamydia effects of interferon-gamma.人鸟苷酸结合蛋白增强干扰素-γ的抗衣原体作用。
PLoS One. 2009 Aug 4;4(8):e6499. doi: 10.1371/journal.pone.0006499.
10
Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I.甲型流感病毒的NS1蛋白靶向泛素连接酶TRIM25，以逃避宿主病毒RNA传感器RIG-I的识别。
Cell Host Microbe. 2009 May 8;5(5):439-49. doi: 10.1016/j.chom.2009.04.006.