Fustinoni S, Polledri E, Mercadante R, Rubino F, Colosio C, Moretto A
Dipartimento di Scienze Cliniche e di Comunità, Università degli Studi di Milano e Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Via S.Barnaba, 8 20122 Milano, Italia.
G Ital Med Lav Ergon. 2012 Jul-Sep;34(3 Suppl):423-4.
Tebuconazole (TEB) is a fungicide widely used in vineyards. This work aimed at the identification of urinary metabolites of TEB for the biological monitoring of exposure, and to study their kinetics of excretion. Major urinary metabolites of TEB in rats are t-butyl-hydroxy-and-carboxy-tebuconazole (TEB-OH and TEB-COOH). TEB and these metabolites were determined in urine samples of 5 wine growers who collected each void before (24 hours), during and after (48 hours) TEB application. These chemicals were found in 95%, 100% and 100% of the samples with levels of < 1.5-13.4 microg/L for TEB, 5.2-749 microg/L for TEB-OH e 2.8-234 microg/l for TEB-COOH. TEB-OH is the major metabolite of TEB, its concentration increases at the end of exposure and peaks after 16-24 hours. TEB-COOH has similar pattern. TEB-OH and TEB -COOH are promising candidates for biological monitoring of TEB exposure; preliminary results suggest the day after the application as the best sampling time.
戊唑醇（TEB）是一种广泛应用于葡萄园的杀菌剂。这项工作旨在鉴定TEB的尿液代谢物，用于生物监测暴露情况，并研究其排泄动力学。大鼠尿液中TEB的主要代谢物是叔丁基羟基和羧基戊唑醇（TEB-OH和TEB-COOH）。在5名葡萄种植者的尿液样本中测定了TEB及其这些代谢物，这些种植者在施用TEB之前（24小时）、期间和之后（48小时）收集每次排尿的尿液。在95%、100%和100%的样本中发现了这些化学物质，TEB的含量为<1.5 - 13.4微克/升，TEB-OH为5.2 - 749微克/升，TEB-COOH为2.8 - 234微克/升。TEB-OH是TEB的主要代谢物，其浓度在暴露结束时增加，并在16 - 24小时后达到峰值。TEB-COOH具有类似的模式。TEB-OH和TEB-COOH有望作为TEB暴露生物监测的候选物；初步结果表明施用后的第二天是最佳采样时间。
