复发性口腔溃疡的炎症因子基因多态性

Inflammatory factors gene polymorphism in recurrent oral ulceration.

机构信息

Center for DNA Typing, Fourth Military Medical University, Xi'an, China.

出版信息

J Oral Pathol Med. 2013 Aug;42(7):528-34. doi: 10.1111/jop.12048. Epub 2013 Feb 14.

DOI:10.1111/jop.12048

PMID:23406059

Abstract

BACKGROUND

Some inflammatory factors play an important role in recurrent oral ulceration (ROU). The genetics mechanism of expression level of inflammatory factors is not clear in ROU, but from genetics the expression level of inflammatory factors at least partly depend on the gene polymorphisms. Therfore, we decided to investigate inflammatory factors gene polymorphism and its association with the susceptibility of recurrent oral ulceration in Chinese.

METHODS

Genomic DNA was obtained from 42 subjects with recurrent oral ulceration, 86 subjects of healthy control individuals.Genotypes and alleles of 10 genes and 17 polymorphisms sites were analyzed by Mass-ARRAY Analyzer method. Then, the differences in distribution of each genotype and allele were compared.

RESULTS

The statistical differences in distribution of TNF-α (rs1800629 and rs1800630) genotype and allele were observed among the groups with recurrent oral ulceration and healthy control individuals (P < 0.01), while VEGFA (rs1570360, rs833061, and rs2010963), EGF (rs4444903), TNF (rs361525), IL10 (rs1800896, rs1800872), IL2 (rs2069762), IL4 (rs2243250), Fas (rs1800682, rs2234767), IL12A (rs2243115, rs568408), IL12B (rs3212227), and IFNG (rs2430561) showed no statistical differences of genotype and allele in controls as compared to those in patients.

CONCLUSIONS

This study suggests that the TNF-α (rs1800629 and rs1800630) genotype is an indicator for the susceptibility of recurrent oral ulceration.

摘要

背景

一些炎症因子在复发性口腔溃疡（ROU）中起着重要作用。ROU 中炎症因子表达水平的遗传机制尚不清楚，但从遗传学角度来看，炎症因子的表达水平至少部分取决于基因多态性。因此，我们决定研究炎症因子基因多态性及其与中国复发性口腔溃疡易感性的关系。

方法

从 42 例复发性口腔溃疡患者和 86 例健康对照个体中提取基因组 DNA。采用 Mass-ARRAY 分析方法分析 10 个基因和 17 个多态性位点的基因型和等位基因。然后，比较各组基因型和等位基因的分布差异。

结果

TNF-α(rs1800629 和 rs1800630) 基因型和等位基因在复发性口腔溃疡组和健康对照组之间的分布存在统计学差异（P<0.01），而 VEGFA(rs1570360、rs833061 和 rs2010963)、EGF(rs4444903)、TNF(rs361525)、IL10(rs1800896、rs1800872)、IL2(rs2069762)、IL4(rs2243250)、Fas(rs1800682、rs2234767)、IL12A(rs2243115、rs568408)、IL12B(rs3212227)和 IFNG(rs2430561) 基因型和等位基因在对照组和患者之间无统计学差异。