School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, 2052, Australia.
Helicobacter. 2013 Aug;18(4):316-20. doi: 10.1111/hel.12045. Epub 2013 Feb 13.
Helicobacter trogontum is a putative enterohepatic pathogen, which following infection of IL-10 knock-out mice, results in severe clinical signs and typhlocolitis.
The pathogenic potential of H. trogontum Type strain LRB 8581 was investigated using proteomics coupled with mass spectrometry to characterize the secretome of H. trogontum and scanning electron microscopy to visualize H. trogontum adherence and invasion.
One hundred and four proteins were identified and bioinformatically predicted to be secreted. Further functional classifications revealed proteins involved in motility, virulence, and colonization factors and the type VI secretion system. Microscopy showed that H. trogontum can adhere to host cells through flagella-microvillus interactions and invade causing a membrane ruffling-like effect and severe cell damage.
This indicated H. trogontum has the ability to adhere to and invade human cells and secrete factors that may contribute to disease development.
幽门螺杆菌 trogontum 是一种假定的肠肝病原体，在感染白细胞介素-10 敲除小鼠后，会导致严重的临床症状和回肠炎。
使用蛋白质组学结合质谱法来描述幽门螺杆菌 trogontum 的分泌组，并使用扫描电子显微镜来观察幽门螺杆菌 trogontum 的黏附和侵袭，从而研究 H. trogontum 型菌株 LRB 8581 的致病潜能。
鉴定出 104 种蛋白质，并预测其具有分泌功能。进一步的功能分类显示，这些蛋白质涉及运动性、毒力和定植因子以及 VI 型分泌系统。显微镜显示，幽门螺杆菌 trogontum 可以通过鞭毛-微绒毛相互作用黏附宿主细胞，并侵入宿主细胞，导致细胞膜起皱样效应和严重的细胞损伤。
这表明幽门螺杆菌 trogontum 具有黏附和侵袭人细胞的能力，并分泌可能有助于疾病发展的因子。
