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叶口蝠科三个属(菊头蝠属、无尾蹄蝠属和筒耳蝠属)线粒体控制区的分子特征与进化

Molecular characteristics and evolution of the mitochondrial control region in three genera (Hipposideridae: Hipposideros Aselliscus and Coelops) of leaf-nosed bats.

作者信息

Sun Keping, Luo Li, Zhang Zhenzhen, Liu Sen, Feng Jiang

机构信息

Jilin Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun 130024, China.

出版信息

Mitochondrial DNA. 2013 Aug;24(4):451-61. doi: 10.3109/19401736.2013.766176. Epub 2013 Feb 13.

DOI:10.3109/19401736.2013.766176
PMID:23406568
Abstract

The mitochondrial control region (CR) was sequenced for three genera of Hipposideridae to give a detailed overview of its features. The CR of leaf-nosed bats (1288-1560 bp) was divided into three domains like that of other mammals. In addition to the common conserved blocks (ETAS1, ETAS2, F-B boxes, CSB1, CSB2, and CSB3) found in all species, a CSB1-like element was also detected in the conserved sequence blocks (CSB). Repeated motifs were examined in the ETAS of Aselliscus stoliczkanus (26 bp) and Hipposideros bicolor (80 bp) and were present in the CSB of all individuals (6, 8, 16, and 20 bp). Phylogenetic reconstructions using the CR sequences indicated that the phylogenetic relationships among Hipposideros species were consistent with the results of other molecular and phenetic analyses. Aselliscus and Coelops had a closer relationship. But the central domain could not be used for phylogenetic analyses at family and genus levels due to its high conservation.

摘要

对菊头蝠科三个属的线粒体控制区(CR)进行了测序,以详细概述其特征。叶鼻蝠的CR(1288 - 1560 bp)与其他哺乳动物一样分为三个结构域。除了在所有物种中都发现的常见保守区域(ETAS1、ETAS2、F - B框、CSB1、CSB2和CSB3)外,在保守序列块(CSB)中还检测到一个类似CSB1的元件。对斯氏菊头蝠(26 bp)和双色菊头蝠(80 bp)的ETAS中的重复基序进行了检测,并且在所有个体的CSB中都存在(6、8、16和20 bp)。使用CR序列进行的系统发育重建表明,菊头蝠属物种之间的系统发育关系与其他分子和表型分析的结果一致。斯氏菊头蝠属和筒菊头蝠属关系更近。但由于中央结构域高度保守,不能用于科级和属级的系统发育分析。

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