Federal Scientific Center of the East Asia Terrestrial Biodiversity Far Eastern Branch of Russian Academy of Sciences, 159 Prospect Stoletiya St., Vladivostok, 690022, Russia.
Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, 6 Volodarskogo St., Tyumen, 625003, Russia.
Mol Biol Rep. 2023 Jun;50(6):5137-5146. doi: 10.1007/s11033-023-08468-4. Epub 2023 Apr 28.
Tandem repeats in mitochondrial DNA control region are known to different animal taxa, including bat species of the family Vespertilionidae. The long R1-repeats in the bat ETAS-domain are often presented in a variable copy number and may exhibit both inter-individual and intra-individual sequence diversity. The function of repeats in the control region is still unclear, but it has been shown that repetitive sequences in some animal groups (shrews, cats and sheep) may include parts of ETAS1 and ETAS2 conservative blocks of mitochondrial DNA.
Analysis of the control region sequences for 31 Myotis petax specimens allowed the identification of the inter-individual variability and clarification of the composition of the R1-repeats. The copy number of the R1-repeats varies from 4 to 7 in individuals. The specimens examined do not exhibit a size heteroplasmy previously described for Myotis species. The unusual short 30 bp R1-repeats have been detected in M. petax for the first time. The ten specimens from Amur Region and Primorsky Territory have one or two copies of these additional repeats.
It was determined that the R1-repeats in M. petax control region consist of parts of the ETAS1 and ETAS2 blocks. The origin of the additional repeats seems to be related to the 51 bp deletion in the central part of the R1-repeat unit and subsequent duplication. Comparison of repetitive sequences in the control region of closely-related Myotis species identified the occurrence of incomplete repeats also resulting from the short deletions, but distinct from additional repeats of M. petax.
串联重复序列存在于线粒体 DNA 控制区，这在包括翼手目蝙蝠科在内的许多动物分类中是已知的。蝙蝠 ETAS 结构域中的长 R1 重复序列通常以可变拷贝数存在，并表现出个体间和个体内的序列多样性。控制区重复序列的功能尚不清楚，但已经表明，在一些动物群体（鼩鼱、猫和羊）中，重复序列可能包含 ETAS1 和 ETAS2 保守区的部分序列。
对 31 只 Myotis petax 标本的控制区序列进行分析，确定了个体间的变异性，并阐明了 R1 重复序列的组成。R1 重复序列的拷贝数在个体间从 4 到 7 不等。研究样本未表现出先前在 Myotis 种中描述的大小异质性。首次在 M. petax 中检测到不寻常的短 30bp R1 重复序列。来自阿穆尔地区和滨海边疆区的 10 个标本具有一个或两个这种额外重复序列的拷贝。
确定了 M. petax 控制区 R1 重复序列由 ETAS1 和 ETAS2 区的部分序列组成。额外重复序列的起源似乎与 R1 重复单元中心部分的 51bp 缺失以及随后的重复有关。对亲缘关系密切的 Myotis 种控制区重复序列的比较确定了不完全重复序列的发生，这些序列也源于短缺失，但与 M. petax 的额外重复序列不同。
