Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, PL 60-631 Poznań, Poland.
Pharmacol Rep. 2012;64(6):1547-53. doi: 10.1016/s1734-1140(12)70952-0.
The aim of the conducted studies was to evaluate the effect of 4-methylpyrazole, increasingly used in detoxifying treatments after ethylene glycol poisoning, on the activity of some antioxidant enzymes and lipid peroxidation formation in the liver of rats after experimental co-exposure to ethylene glycol and ethyl alcohol.
The trials were conducted on adult male Wistar rats. Ethylene glycol (EG) at the dose of 3.83 g/kg bw and ethyl alcohol (EA) at the dose of 1 g/kg bw were administered po, and 4-methylpyrazole (4-MP) at the dose of 0.01 g/kg bw was administered ip. Parameters of antioxidant balance were evaluated in hepatic cytosol, including the activity of the following enzymes: glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation level (TBARS).
The results suggest that evaluation of the effects of administrated 4-MP after co-exposure to EG and EA in the liver revealed statistically significant changes on antioxidant enzyme system and malondialdehyde formation.
The changes in biomarkers activity indicate a greater production of free radicals which exceeds the capability of antioxidant system, appearing with oxidative stress in the group of animals treated by 4-MP combined with EG and EA.
本研究旨在评估 4-甲基吡唑（一种常用于乙二醇中毒解毒治疗的药物）对同时接触乙二醇和乙醇的大鼠肝脏中某些抗氧化酶活性和脂质过氧化形成的影响。
试验在成年雄性 Wistar 大鼠中进行。乙二醇（EG）剂量为 3.83 g/kg bw，乙醇（EA）剂量为 1 g/kg bw，给予口服；4-甲基吡唑（4-MP）剂量为 0.01 g/kg bw，给予腹腔注射。评估肝胞质中抗氧化平衡的参数，包括以下酶的活性：谷胱甘肽 S-转移酶（GST）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPx）和脂质过氧化水平（TBARS）。
结果表明，在同时接触 EG 和 EA 后给予 4-MP 的实验中，肝脏中抗氧化酶系统和丙二醛形成的评估显示出统计学上的显著变化。
生物标志物活性的变化表明自由基的产生增加，超过了抗氧化系统的能力，在同时接受 EG 和 EA 治疗的动物组中出现了氧化应激。
