从活体斑马鱼侧线毛细胞的膜片钳记录。
Patch-clamp recordings from lateral line neuromast hair cells of the living zebrafish.
机构信息
Department of Otolaryngology, Stanford University School of Medicine, Stanford, California 94305, USA.
出版信息
J Neurosci. 2013 Feb 13;33(7):3131-4. doi: 10.1523/JNEUROSCI.4265-12.2013.
Abstract
Zebrafish are popular models for biological discovery. For investigators of the auditory and vestibular periphery, manipulations of hair cell and synaptic mechanisms have relied on inferences from extracellular recordings of physiological activity. We now provide data showing that hair cells and supporting cells of the lateral line can be directly patch-clamped, providing the first recordings of ionic channel activity, synaptic vesicle release, and gap junctional coupling in the neuromasts of living fish. Such capabilities will allow more detailed understanding of mechano-sensation of the zebrafish.
摘要
斑马鱼是生物学发现的常用模式生物。对于听觉和前庭外周的研究人员来说，对毛细胞和突触机制的操作依赖于对生理活性的细胞外记录的推断。我们现在提供的数据表明，侧线的毛细胞和支持细胞可以直接进行膜片钳记录，从而提供了活体鱼鱼鳍感受器官中离子通道活动、突触小泡释放和缝隙连接偶联的首次记录。这些能力将使我们能够更详细地了解斑马鱼的机械感觉。
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