血清白蛋白在不同自由流体电泳仪器中的等速电泳区带形成

Isotachophoretic zone formation of serum albumin in different free fluid electrophoresis instruments.

作者信息

Thormann W, Firestone M A, Sloan J E, Long T D, Mosher R A

机构信息

Center for Separation Science, University of Arizona, Tucson.

出版信息

Electrophoresis. 1990 Apr;11(4):298-304. doi: 10.1002/elps.1150110405.

DOI:10.1002/elps.1150110405

PMID:2340824

Abstract

The isotachophoretic behavior of a model protein, serum albumin, was examined (i) by computer simulation, (ii) by capillary isotachophoresis in HPE 100 and Tachophor 2127, (iii) by continuous flow isotachophoresis in Elphor VaP 22 and the BIO-STREAM Separator and (iv) by recycling isotachophoresis in an apparatus of our own design. Variations in monitored zone shapes can be explained by differences in engineering aspects and fluid stabilization principles of the instruments.

摘要

对一种模型蛋白质——血清白蛋白的等速电泳行为进行了如下研究：（i）通过计算机模拟；（ii）在HPE 100和Tachophor 2127中进行毛细管等速电泳；（iii）在Elphor VaP 22和BIO-STREAM分离器中进行连续流动等速电泳；（iv）在我们自行设计的仪器中进行循环等速电泳。监测区域形状的变化可以通过仪器的工程方面和流体稳定原理的差异来解释。