Reijenga J, Kasicka V
Laboratory of Instrumental Analysis, University of Technology, Eindhoven, The Netherlands.
Electrophoresis. 1998 Jul;19(10):1601-5. doi: 10.1002/elps.1150191014.
Discontinuities of pH at zone boundaries of strong ion sample zones in isotachophoretic systems with poorly buffering leading electrolytes have been discovered by dynamic computer simulations of isotachophoretic separations with uncommon electrolyte systems. When using a salt solution containing a strong cation (anion) as leading ion and a weak anion (cation) as counterion for the isotachophoretic separation of strong cations (anions), severe pH discontinuities are present at zone boundaries of these cations (anions). The magnitude of these pH discontinuities has been investigated as a function of several parameters, namely counter ion/leading ion concentration ratio of the leading electrolyte, pK values, and mobilities of sample ions and counterion.
通过对采用特殊电解质系统的等速电泳分离进行动态计算机模拟，发现在缓冲能力较差的前导电解质的等速电泳系统中，强离子样品区带边界处存在pH值的不连续性。当使用含有强阳离子（阴离子）作为前导离子和弱阴离子（阳离子）作为抗衡离子的盐溶液对等速电泳分离强阳离子（阴离子）时，这些阳离子（阴离子）的区带边界处存在严重的pH值不连续性。已研究了这些pH值不连续性的大小与几个参数的函数关系，即前导电解质的抗衡离子/前导离子浓度比、pK值以及样品离子和抗衡离子的迁移率。
