Lian L H, Lau T P, Lee V L, Lee W S, Hilmi I, Goh K L, Chua K H
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Genet Mol Res. 2013 Jan 24;12(1):167-74. doi: 10.4238/2013.January.24.9.
This study aimed to investigate the potential association of TYK2 and STAT3 genes with the susceptibility to Crohn's disease (CD) among Malaysians. DNA samples were obtained from 80 CD patients and 100 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism methods were employed for genotyping, followed by statistical analysis. In our current study, none of the single nucleotide polymorphisms of either TYK2 or STAT3 was statistically associated with the susceptibility to CD in our local population (P > 0.05). In contrast, there was a statistically significant association between the G/G homozygotes of the STAT3 rs2293152 and the healthy control group (χ(2) = 6.229, P < 0.05). In conclusion, our study does not support the role of the TYK2 and STAT3 genes influencing CD susceptibility.
本研究旨在调查马来西亚人中酪氨酸激酶2（TYK2）和信号转导与转录激活因子3（STAT3）基因与克罗恩病（CD）易感性之间的潜在关联。从80例CD患者和100例健康对照者中获取DNA样本。采用聚合酶链反应-限制性片段长度多态性方法进行基因分型，随后进行统计分析。在我们目前的研究中，TYK2或STAT3的单核苷酸多态性均与我们当地人群的CD易感性无统计学关联（P>0.05）。相比之下，STAT3 rs2293152的G/G纯合子与健康对照组之间存在统计学显著关联（χ(2)=6.229，P<0.05）。总之，我们的研究不支持TYK2和STAT3基因影响CD易感性的作用。
