Department of Otolaryngology-Head and Neck Surgery, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.
PLoS One. 2013;8(2):e56323. doi: 10.1371/journal.pone.0056323. Epub 2013 Feb 7.
Glucocorticoids (GCs) are widely used to treat sudden sensorineural hearing loss (SSNHL) and significantly improve hearing. However, GC insensitivity has been observed in some patients of SSNHL.
To study the correlation between GR expression in peripheral blood mononuclear cells (PBMCs) and in the cochlea of guinea pigs at mRNA and protein levels.
One group of guinea pigs received dexamethasone (10 mg/kg/day) intraperitoneally for 7 consecutive days (dexamethasone group), and another group of guinea pigs received normal saline (control group). Real time PCR and Western blotting were used to detect the expression of GR mRNA and GR protein in PBMCs and the cochleae.
The GR mRNA and GR protein were detected in both PBMCs and the cochlear tissue of guinea pigs. GR mRNA and GR protein levels in PBMCs were positively correlated with those in the cochlea. The expression of GR mRNA and GR protein was significantly increased in the dexamethasone group compared to the control group.
Levels of GR mRNA and GR protein in the PBMCs were positively correlated with those in the cochlea of guinea pigs. Systemic dexamethasone treatment can significantly up-regulate GR expression in PBMCs and in the cochlea. Measurement of the GR level in PBMCs could be used as an indicator of GR level in the cochlea.
糖皮质激素（GCs）广泛用于治疗突发性聋，并显著改善听力。然而，一些突发性聋患者存在 GC 不敏感现象。
研究豚鼠外周血单个核细胞（PBMC）和耳蜗中 GR 表达在 mRNA 和蛋白水平上的相关性。
一组豚鼠连续 7 天腹腔内给予地塞米松（10 mg/kg/天）（地塞米松组），另一组豚鼠给予生理盐水（对照组）。实时 PCR 和 Western blot 用于检测 PBMC 和耳蜗中 GR mRNA 和 GR 蛋白的表达。
豚鼠 PBMC 和耳蜗组织中均检测到 GR mRNA 和 GR 蛋白。GR mRNA 和 GR 蛋白在 PBMC 中的表达与耳蜗中的表达呈正相关。与对照组相比，地塞米松组 GR mRNA 和 GR 蛋白的表达显著增加。
豚鼠 PBMC 中 GR mRNA 和 GR 蛋白的水平与耳蜗中的水平呈正相关。全身地塞米松治疗可显著上调 PBMC 和耳蜗中 GR 的表达。PBMC 中 GR 水平的测定可作为耳蜗中 GR 水平的指标。
