O'Neill I E, Houston B, Goddard C
Department of Molecular Endocrinology, AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian, U.K.
Mol Cell Endocrinol. 1990 Mar 26;70(1):41-7. doi: 10.1016/0303-7207(90)90057-f.
Extracts of conditioned media from primary cultures of chicken hepatocytes stimulated [3H]thymidine incorporation into chick embryo fibroblasts demonstrating that the cells released mitogen(s) into the medium. There was also a simultaneous release of insulin-like growth factor I (IGF-I) immunoreactivity into the medium. Acid chromatography of freeze-dried extracts of conditioned media by high performance gel permeation chromatography demonstrated that IGF-I immunoreactivity eluted in a major peak with a molecular weight of 7500 Da and a minor peak with a molecular weight of 55,000 Da. The release of IGF-I immunoreactivity was increased by pituitary-derived chicken growth hormone (cGH) in a dose-dependent manner with half-maximum stimulation occurring at a cGH concentration of 40 ng/ml and maximum stimulation at cGH concentrations greater than 800 ng/ml. These results demonstrate that cultured chicken hepatocytes produce IGF-I and that this can be stimulated by cGH in vitro.
鸡肝细胞原代培养的条件培养基提取物刺激[3H]胸腺嘧啶核苷掺入鸡胚成纤维细胞，表明细胞向培养基中释放了促有丝分裂原。同时也有胰岛素样生长因子I（IGF-I）免疫反应性释放到培养基中。通过高效凝胶渗透色谱对条件培养基的冻干提取物进行酸性色谱分析表明，IGF-I免疫反应性在一个分子量为7500 Da的主峰和一个分子量为55000 Da的次峰中洗脱。垂体来源的鸡生长激素（cGH）以剂量依赖的方式增加IGF-I免疫反应性的释放，在cGH浓度为40 ng/ml时出现半数最大刺激，在cGH浓度大于800 ng/ml时出现最大刺激。这些结果表明，培养的鸡肝细胞产生IGF-I，并且在体外可被cGH刺激。
