Department of Food Science and Technology, Carbohydrate Bioproduct Research Center, Sejong University, Gunja-dong 98, Gwangjin-gu, Seoul 143-747, Republic of Korea.
Food Chem. 2013 Jun 1;138(2-3):966-75. doi: 10.1016/j.foodchem.2012.11.028. Epub 2012 Nov 15.
Structural modification of rice and barley starches with Neisseria polysaccharea amylosucrase (NpAS) was conducted, and relationship between structural characteristics and resistant starch (RS) contents of NpAS-treated starches was investigated. Pre-gelatinised rice and barley starches were treated with NpAS. NpAS-treated starches were characterised with respect to morphology, X-ray diffraction pattern, amylopectin branch-chain distribution, and RS content, and their structural characteristics were correlated to RS contents. Regardless of amylose contents of native starches, NpAS-treated (relative to native) starches possessed lower and higher proportions of shorter (DP 6-12) and intermediate (DP 13-36) amylopectin (AP) branch-chains, respectively. RS contents were higher for NpAS-treated starches relative to native starches, and maximum RS contents were obtained for NpAS-treated starches of waxy rice and barley genotypes. Amylose contents were not associated with RS contents of NpAS-treated starches. However, shorter and intermediate AP branch-chain portions were negatively and positively correlated to RS contents of NpAS-treated starches, respectively.
用 Neisseria polysaccharea 淀粉蔗糖酶（NpAS）对水稻和大麦淀粉进行结构修饰，并研究 NpAS 处理淀粉的结构特性与抗性淀粉（RS）含量之间的关系。将预胶化的大米和大麦淀粉用 NpAS 进行处理。用形态学、X 射线衍射图谱、支链淀粉分支链分布和 RS 含量对 NpAS 处理的淀粉进行了表征，并将其结构特性与 RS 含量相关联。无论原淀粉的直链淀粉含量如何，NpAS 处理的（相对于原淀粉）淀粉中分别具有较短（DP 6-12）和中等（DP 13-36）支链淀粉（AP）分支链的比例较低和较高。与原淀粉相比，NpAS 处理的淀粉的 RS 含量更高，并且糯稻和大麦基因型的 NpAS 处理淀粉的 RS 含量最高。直链淀粉含量与 NpAS 处理淀粉的 RS 含量无关。然而，较短和中等的 AP 分支链部分与 NpAS 处理淀粉的 RS 含量呈负相关和正相关。
