解偶联蛋白2和3基因多态性及能量消耗在印度尼西亚肥胖儿童中的作用

The role of uncoupling protein 2 and 3 genes polymorphism and energy expenditure in obese Indonesian children.

作者信息

Mexitalia Maria, Yamauchi Taro, Utari Agustini, Sjarif Damayanti R, Subagio Hertanto W, Soemantri Agustinus, Ishida Takafumi

机构信息

Department of Pediatrics, Faculty of Medicine, Diponegoro University, Dr. Kariadi Hospital, Dr. Sutomo 16-18 Semarang Central Java 50231, Semarang, Indonesia.

出版信息

J Pediatr Endocrinol Metab. 2013;26(5-6):441-7. doi: 10.1515/jpem-2012-0311.

DOI:10.1515/jpem-2012-0311

PMID:23412863

Abstract

AIM

Uncoupling protein (UCP) genes, which may contribute to energy metabolism in mitochondria, may be involved in the pathogenesis of obesity. We analyzed the differences in energy expenditure between single nucleotide polymorphisms (SNPs) UCP3-55C/T, UCP3 Y210Y, and UCP2 A55V among Indonesian children.

METHODS

The study included 76 schoolchildren (36 obese and 40 healthy; mean age, 12.8 years) in Semarang, Indonesia. Body composition was measured by bioelectrical impedance analysis; resting energy expenditure (REE) by indirect calorimetry; physical activity by uniaxial accelerometer; and total energy expenditure (TEE) by the equations extrapolated from REE and physical activity. UCP3-55C/T, UCP3 Y210Y, and UCP2 A55V were examined by restriction length fragment polymorphism analysis.

RESULTS

The TEE of the subjects with the T/T genotype at UCP3-55C/T after adjusting for fat-free mass (63.2±7.2 kcal/kg/day) and T/T at UCP2 A55V (62.8±5.6 kcal/kg/day) was lower than that of the subjects with the C/C and C/T genotypes (p<0.05). The REE of the subjects with these T/T genotypes tended to be lower than that of the subjects with C/C and C/T (p≥0.05). No significant differences in REE or TEE were found between the UCP3 Y210Y genotypes.

CONCLUSIONS

The subjects with the T/T genotypes of UCP3-55C/T or UCP2 A55V had lower TEE than those with other genotypes.

摘要

目的

解偶联蛋白（UCP）基因可能参与线粒体能量代谢，可能与肥胖发病机制有关。我们分析了印度尼西亚儿童中解偶联蛋白3（UCP3）基因单核苷酸多态性（SNP）-55C/T、UCP3 Y210Y以及解偶联蛋白2（UCP2）基因A55V在能量消耗方面的差异。

方法

该研究纳入了印度尼西亚三宝垄市的76名学童（36名肥胖儿童和40名健康儿童；平均年龄12.8岁）。通过生物电阻抗分析法测量身体成分；采用间接测热法测量静息能量消耗（REE）；使用单轴加速度计测量身体活动情况；根据REE和身体活动情况推算出的公式计算总能量消耗（TEE）。通过限制性片段长度多态性分析检测UCP3 - 55C/T、UCP3 Y210Y以及UCP2 A55V。

结果

在校正去脂体重后，UCP3 - 55C/T基因T/T基因型受试者的TEE（63.2±7.2千卡/千克/天）以及UCP2 A55V基因T/T基因型受试者的TEE（62.8±5.6千卡/千克/天）低于C/C和C/T基因型受试者（p<0.05）。这些T/T基因型受试者的REE也倾向于低于C/C和C/T基因型受试者（p≥0.05）。UCP3 Y210Y基因各基因型之间的REE或TEE未发现显著差异。