货物大小和形状对细菌 Tat 转运酶转运效率的影响。
Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase.
机构信息
Department of Molecular and Cellular Medicine, College of Medicine, The Texas A&M Health Science Center, 1114 TAMU, College Station, TX 77843, USA.
出版信息
FEBS Lett. 2013 Apr 2;587(7):912-6. doi: 10.1016/j.febslet.2013.02.015. Epub 2013 Feb 16.
Abstract
The Tat machinery translocates fully-folded and oligomeric substrates. The passage of large, bulky cargos across an ion-tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre-SufI as a base. Four (of 17) cargos transported with significant (>20% of wild-type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability.
摘要
Tat 机器将完全折叠和寡聚化的底物转运。大而笨重的货物穿过离子紧密的膜的通道表明需要匹配孔和货物的大小，因此 Tat 运输效率可能取决于货物的大小和形状。使用天然 Tat 底物 pre-SufI 作为基础，生成了一系列不同大小和形状的货物。其中四个（占野生型的 17%）以显著的效率（>20%）进行转运。这些结果表明货物的大小和形状显著影响 Tat 的可运输性。
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