miR-154 通过靶向 CCND2 抑制前列腺癌细胞增殖。
miR-154 inhibits prostate cancer cell proliferation by targeting CCND2.
机构信息
State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
出版信息
Urol Oncol. 2014 Jan;32(1):31.e9-16. doi: 10.1016/j.urolonc.2012.11.013. Epub 2013 Feb 19.
BACKGROUND
Research has shown reduced expression levels of miR-154 in prostate cancer (CaP). However, the function and molecular mechanisms of miR-154 in this cancer type remains unknown.
OBJECTIVE
The aims of this study were to examine the functional significance of miR-154 in CaP cells and to identify the novel molecular targets regulated by miR-154.
MATERIALS AND METHODS
miR-154 expression significantly decreased in primary CaP samples compared with nonmalignant samples measured by quantitative reverse transcription polymerase chain reaction. Restoration of miR-154 lowered the potential of CaP cell lines to grow and proliferate in vitro evaluated by CCK-8 assay, colony formation assay, and flow cytometry. miR-154 down-regulated the expression of CCND2 by binding to its 3'-untranslated region by luciferase reporter assay.
CONCLUSIONS
miR-154 plays a prominent role in CaP proliferation by suppressing CCND2, and it may provide a new approach to the treatment of CaP.
背景
研究表明，前列腺癌（CaP）中 miR-154 的表达水平降低。然而，miR-154 在这种癌症类型中的功能和分子机制尚不清楚。
目的
本研究旨在探讨 miR-154 在 CaP 细胞中的功能意义，并鉴定由 miR-154 调控的新的分子靶标。
材料和方法
通过定量逆转录聚合酶链反应测量，miR-154 在原发性 CaP 样本中的表达水平明显低于非恶性样本。通过 CCK-8 测定、集落形成测定和流式细胞术评估，miR-154 的恢复降低了 CaP 细胞系在体外生长和增殖的潜力。通过荧光素酶报告基因测定证实，miR-154 通过结合其 3'-非翻译区下调 CCND2 的表达。
结论
miR-154 通过抑制 CCND2 在 CaP 增殖中发挥重要作用，它可能为 CaP 的治疗提供新的方法。
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