Kadekaro M, Nermo-Lindquist E, Terrell M L, Kelly S M, Freeman S, Gross P M, Eisenberg H M
Division of Neurosurgery, University of Texas Medical Branch, Galveston 77550.
Regul Pept. 1990 Apr 24;28(2):153-9. doi: 10.1016/0167-0115(90)90014-n.
The pituitary neural lobe of homozygous Brattleboro rats has high rates of glucose utilization not affected by chronic treatment with exogenous vasopressin, despite attenuation of polydipsia and polyuria. We evaluated whether this effect may result from the inability of vasopressin to affect the hypothalamo-neurohypophysial metabolism or from the development of resistance to chronic vasopressin treatment. We used the [14C]deoxyglucose method to compare 28-h effects of vasopressin treatment (5 U/kg, i.m., twice a day) with that of desmopressin (100 micrograms/kg, i.p., once a day), a long-lasting antidiuretic hormone, on glucose utilization of the hypothalamo-neurohypophysial system and related structures in conscious homozygous Brattleboro rats. Vasopressin and desmopressin reduced water intake, plasma osmolality and plasma Na+ concentration similarly. Vasopressin decreased glucose utilization in the supraoptic nucleus, subfornical organ and median preoptic nucleus, but did not alter activity in the paraventricular nucleus and neural lobe. Desmopressin decreased glucose utilization in all these structures. The results indicate that desmopressin has a more potent inhibitory action on the hypothalamo-neurohypophysial system than vasopressin over this short duration of treatment. The lack of response in the neural lobe from chronic treatment with vasopressin seems to be due to its inability to affect the paraventricular nucleus metabolism. The maintenance of metabolic activity in the paraventricular nucleus of vasopressin-treated Brattleboro rats suggests that this structure contributes importantly to the metabolism of neural lobe.
纯合布拉特洛维大鼠的垂体神经叶具有较高的葡萄糖利用率，尽管多饮多尿症状有所减轻，但外源性加压素的长期治疗对此并无影响。我们评估了这种效应是由于加压素无法影响下丘脑 - 神经垂体代谢，还是由于对加压素长期治疗产生了抗性。我们采用[14C]脱氧葡萄糖法，比较了加压素治疗（5 U/kg，肌肉注射，每日两次）与去氨加压素（100 μg/kg，腹腔注射，每日一次，一种长效抗利尿激素）对清醒纯合布拉特洛维大鼠下丘脑 - 神经垂体系统及相关结构葡萄糖利用的28小时影响。加压素和去氨加压素同样降低了水摄入量、血浆渗透压和血浆钠浓度。加压素降低了视上核、穹窿下器官和视前正中核的葡萄糖利用率，但未改变室旁核和神经叶的活性。去氨加压素降低了所有这些结构的葡萄糖利用率。结果表明，在这种短期治疗中，去氨加压素对下丘脑 - 神经垂体系统的抑制作用比加压素更强。长期用加压素治疗后神经叶缺乏反应似乎是由于其无法影响室旁核代谢。加压素治疗的布拉特洛维大鼠室旁核代谢活性的维持表明，该结构对神经叶的代谢有重要贡献。
