Yuasa H, Yamanaka H, Imai K, Mashimo T, Kohno H
Department of Urology, Gunma University School of Medicine.
Hinyokika Kiyo. 1990 Feb;36(2):121-5.
The purification of 45 KDa EMBP and the production of monospecific anti-serum is described. 45 KDa EMBP was purified by relatively simple methods using ion exchange HPLC (TSK-GEL DEAE-5PW column) and size exclusion HPLC (TSK-GEL G3000SW column). The results clearly demonstrated the speed and simplicity of the method using these columns, compared to previously-described methods for purification of 45 KDa EMBP.
本文描述了45 kDa EMBP的纯化及单特异性抗血清的制备。采用离子交换高效液相色谱法（TSK-GEL DEAE-5PW柱）和尺寸排阻高效液相色谱法（TSK-GEL G3000SW柱），通过相对简单的方法对45 kDa EMBP进行了纯化。结果清楚地表明，与先前描述的45 kDa EMBP纯化方法相比，使用这些色谱柱的方法具有速度快和操作简单的特点。
