[三碘甲状腺原氨酸不会增加雌性大鼠骨髓间充质干细胞中因年龄增长而降低的成骨分化能力]
[Triiodothyronine does not increase osteogenic differentiation reduced by age in bone marrow mesenchymal stem cells of female rats].
作者信息
Boeloni Jankerle Neves, Ocarino Natalia Melo, Goes Alfredo Miranda, Serakides Rogéria
机构信息
Núcleo de Células-tronco e Terapia Celular, Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brasil.
出版信息
Arq Bras Endocrinol Metabol. 2013 Feb;57(1):62-70. doi: 10.1590/s0004-27302013000100009.
OBJECTIVE
To examine if triiodothyronine (T3) increases osteogenic differentiation in bone marrow mesenchymal stem cells (BMMSCs) of adult rats compared with young rats.
MATERIALS AND METHODS
BMMSCs were cultured in osteogenic medium and distributed into six groups: 1) BMMSCs of young rats; 2) BMMSCs of adult rats; 3, 4, 5 and 6) BMMSCs of adult rats with T3 (0.01, 1, 100 to 1000 nM). We analyzed alkaline phosphatase activity, dimethylthiazol (MTT) conversion, and collagen synthesis at 7, 14, and 21 days, and percentage of cells per field and number of mineralized nodules at 21 days of differentiation.
RESULTS
T3 reduced MTT conversion, alkaline phosphatase activity, collagen synthesis, and the synthesis of mineralizalized nodules in at least one of the doses and periods studied (p < 0.05). Values were lower when compared with young and adult rats BMMSCs (p < 0.05) without T3.
CONCLUSION
T3 has a negative effect on the factors involved in osteogenic differentiation of BMMSC from adult rats.
目的
研究与幼鼠相比，三碘甲状腺原氨酸（T3）是否能增强成年大鼠骨髓间充质干细胞（BMMSC）的成骨分化。
材料与方法
将BMMSC培养于成骨培养基中，并分为六组：1）幼鼠BMMSC；2）成年大鼠BMMSC；3、4、5和6）添加T3（0.01、1、100至1000 nM）的成年大鼠BMMSC。在第7、14和21天分析碱性磷酸酶活性、二甲基噻唑（MTT）转化率和胶原蛋白合成，并在分化21天时分析每视野细胞百分比和矿化结节数量。
结果
在所研究的至少一个剂量和时间段内，T3降低了MTT转化率、碱性磷酸酶活性、胶原蛋白合成以及矿化结节的合成（p < 0.05）。与未添加T3的幼鼠和成年大鼠BMMSC相比，这些值更低（p < 0.05）。
结论
T3对成年大鼠BMMSC成骨分化相关因子具有负面影响。
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