College of Life Science, Hebei Normal University, Hebei Province, PR China.
Mem Inst Oswaldo Cruz. 2013 Feb;108(1):41-7. doi: 10.1590/s0074-02762013000100007.
Heliconema hainanensis sp. nov. collected from Uroconger lepturus (Richardson) (Anguilliformes: Congridae), Muraenesox cinereus (Forsskål) and Congresox talabonoides (Bleeker) (Anguilliformes: Muraenesocidae) in the South China Sea was described using light and scanning electron microscopy. The new species differs from its congeners by the following morphology: pseudolabia, the number and arrangement of caudal papillae (4 pairs of pedunculate precloacal papillae arranged in 2 groups of 2 and 2 pairs and 6 pairs of pedunculate postcloacal papillae arranged in 4 groups of 1, 2, 1 and 2 pairs), the length of spicules [left spicule 0.51-0.69 mm, right spicule 0.20-0.27 mm, spicule (right:left) ratio 1:2.20-2.69] and the morphology of the female tail tip. In addition, specimens of the new species collected from the three different hosts and specimens of an unidentified species of Heliconema collected from U. lepturus were characterised using molecular methods by sequencing the internal transcribed spacer (ITS) of ribosomal DNA. Analyses and comparison of the ITS sequence of H. hainanensis sp. nov. with Heliconema sp. support the validity of the new species based on morphological observations. An identification key to the species of Heliconema is also provided.
南海采集的来自 Uroconger lepturus (Richardson)（鳗鲡形目：海鳝科）、Muraenesox cinereus (Forsskål) 和 Congresox talabonoides (Bleeker)（鳗鲡形目：海鳝科）的 Heliconema hainanensis sp. nov. 使用光镜和扫描电子显微镜进行了描述。该新种与同属的其他种不同，具有以下形态特征：假唇，尾鳍上的乳突数和排列（4 对具柄的肛前乳突分为 2 组，每组 2 对，6 对具柄的肛后乳突分为 4 组，每组 1、2、1 和 2 对）， 刺的长度[左刺 0.51-0.69 毫米，右刺 0.20-0.27 毫米，刺（右：左）比 1:2.20-2.69]和雌尾端的形态。此外，使用分子方法（核糖体 DNA 的内转录间隔区（ITS）测序）对从三种不同宿主中采集的新种标本和从 U. lepturus 中采集的未鉴定的 Heliconema 种标本进行了特征描述。与 Heliconema sp. 的 ITS 序列分析和比较支持了基于形态观察的新种的有效性。还提供了 Heliconema 种的鉴定钥匙。
