Programa de Pós-Graduação em Ciências Biológicas, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2013 Feb;108(1):110-2. doi: 10.1590/s0074-02762013000100018.
Trichomonas vaginalis and Tritrichomonas foetus are parasitic protists of the human and bovine urogenital tracts, respectively. Several studies have described the cytotoxic effects of trichomonads on urogenital tract epithelial cells. However, little is known about the host cell response against trichomonads. The aim of this study was to determine whether T. foetus and T. vaginalis stimulated the release of the cytokine interleukin (IL)-10 from cultured bovine epithelial cells. To characterise the inflammatory response induced by these parasites, primary cultures of bovine oviduct epithelial cells were exposed to either T. vaginalis or T. foetus. Within 12 h after parasite challenge, supernatants were collected and cytokine production was analysed. Large amounts of IL-10 were detected in the supernatants of cultures that had been stimulated with T. foetus. Interestingly, T. vaginalis induced only a small increase in the release of IL-10 upon exposure to the same bovine cells. Thus, the inflammatory response of the host cell is species-specific. Only T. foetus and not T. vaginalis induced the release of IL-10 by bovine oviduct epithelial cells.
阴道毛滴虫和胎儿三毛滴虫分别是寄生在人体和牛泌尿生殖道的原生动物。有几项研究描述了毛滴虫对泌尿生殖道上皮细胞的细胞毒性作用。然而，对于宿主细胞对毛滴虫的反应知之甚少。本研究旨在确定胎儿三毛滴虫和阴道毛滴虫是否刺激培养的牛上皮细胞释放细胞因子白细胞介素（IL）-10。为了描述这些寄生虫引起的炎症反应，将牛输卵管上皮细胞的原代培养物暴露于阴道毛滴虫或胎儿三毛滴虫。在寄生虫攻击后 12 小时内，收集上清液并分析细胞因子的产生。用胎儿三毛滴虫刺激的培养物上清液中检测到大量的 IL-10。有趣的是，暴露于相同的牛细胞时，阴道毛滴虫仅引起 IL-10 释放的少量增加。因此，宿主细胞的炎症反应具有种属特异性。只有胎儿三毛滴虫而不是阴道毛滴虫诱导牛输卵管上皮细胞释放 IL-10。
