Benchimol Marlene, Dias Angelo Burla, Fontes Reginaldo
Universidade Santa Ursula, Rua Jornalista Orlando Dantas 59, CEP 222-31-010 Botafogo, Rio de Janeiro, RJ, Brazil.
Vet Parasitol. 2006 Sep 10;140(3-4):244-50. doi: 10.1016/j.vetpar.2006.04.011. Epub 2006 May 18.
Tritrichomonas foetus is an extracellular parasite of the reproductive tract in cattle. The mechanism by which T. foetus causes abortion in cattle is largely unknown. There are no studies of infection in the cow oviducts, almost all published papers are related to vagina infection and few articles focusing on the uterus. The aim of the present study was to establish a working model of bovine oviduct epithelial cells and submit these cells to Tritrichomonas foetus interaction. Twenty bovine oviducts were obtained from cows at a commercial abattoir and T. foetus was injected through the isthmus into the oviduct lumen. The whole oviduct was analyzed by scanning and transmission electron microscopy. The results reported here demonstrate that: (1) fresh whole oviducts can be used as a good model to study parasite-host cell interaction; (2) cow oviduct epithelium has been shown to consist of two cell types: ciliated and nonciliated secretory cells, and T. foetus displayed great specificity for the nonciliated cells localized in the deeper oviduct folds; (3) T. foetus adheres as single separate cells, and maintains the flagella externalized; (4) differently from T. vaginalis, T. foetus does not change its shape during the adhesion process; and (5) oviduct cells exhibited morphological characteristics of apoptosis after trichomonadal interaction.
胎儿三毛滴虫是牛生殖道的一种细胞外寄生虫。胎儿三毛滴虫导致牛流产的机制在很大程度上尚不清楚。目前尚无关于母牛输卵管感染的研究，几乎所有已发表的论文都与阴道感染有关，而关注子宫的文章很少。本研究的目的是建立牛输卵管上皮细胞的工作模型，并使这些细胞与胎儿三毛滴虫相互作用。从一家商业屠宰场的母牛身上获取了20条牛输卵管，并通过峡部将胎儿三毛滴虫注入输卵管腔。通过扫描电子显微镜和透射电子显微镜对整个输卵管进行了分析。此处报告的结果表明：（1）新鲜的完整输卵管可作为研究寄生虫与宿主细胞相互作用的良好模型；（2）已证明母牛输卵管上皮由两种细胞类型组成：纤毛细胞和非纤毛分泌细胞，胎儿三毛滴虫对位于输卵管较深褶皱处的非纤毛细胞表现出高度特异性；（3）胎儿三毛滴虫以单个分离的细胞形式附着，并保持鞭毛外露；（4）与阴道毛滴虫不同，胎儿三毛滴虫在附着过程中不会改变其形状；（5）滴虫相互作用后，输卵管细胞表现出凋亡的形态学特征。
