Yuen S N, Folkman J, Weisz P B, Joullie M M, Ewing W R
Department of Surgery, Children's Hospital, Boston, Massachusetts.
Anal Biochem. 1990 Feb 15;185(1):108-11. doi: 10.1016/0003-2697(90)90263-9.
beta-Cyclodextrin tetradecasulfate was found to have a very strong affinity for fibroblast growth factor (FGF) and could substitute for heparin in FGF purification. Basic FGF was purified about 200,000-fold from a rat chondrosarcoma using a method of biaffinity chromatography in which the beta-cyclodextrin tetradecasulfate polymer was mixed with copper-Sepharose. This method takes advantage of the strong affinity of FGF for both beta-cyclodextrin tetradecasulfate and copper.
已发现β-环糊精十四硫酸盐对成纤维细胞生长因子（FGF）具有很强的亲和力，并且在FGF纯化中可替代肝素。使用双亲和色谱法从大鼠软骨肉瘤中纯化碱性FGF约200,000倍，该方法是将β-环糊精十四硫酸盐聚合物与铜-琼脂糖混合。此方法利用了FGF对β-环糊精十四硫酸盐和铜两者的强亲和力。
