Killeen Daniel P, Sansom Catherine E, Lill Ross E, Eason Jocelyn R, Gordon Keith C, Perry Nigel B
Department of Chemistry, University of Otago , P.O. Box 56, Dunedin, New Zealand.
J Agric Food Chem. 2013 Mar 20;61(11):2701-8. doi: 10.1021/jf3053669. Epub 2013 Mar 11.
Rapid quantitative near-infrared Fourier transform Raman analyses of the key phytonutrients in carrots, polyacetylenes and carotenoids, are reported here for the first time. Solvent extracts of 31 carrot lines were analyzed for these phytonutrients by conventional methods, polyacetylenes by GC-FID and carotenoids by visible spectrophotometry. Carotenoid concentrations were 0-5586 μg g(-1) dry weight (DW). Polyacetylene concentrations were 74-4846 μg g(-1) DW, highest in wild carrots. The polyacetylenes were falcarinol, 6-1237 μg g(-1) DW; falcarindiol, 42-3475 μg g(-1) DW; and falcarindiol 3-acetate, 27-649 μg g(-1) DW. Strong Raman bands for carotenoids gave good correlation to results by visible spectrophotometry. A chemometric model capable of quantitating carotenoids from Raman data was developed. A classification model for rapidly distinguishing carrots with high and low polyacetylene (limit of detection = 1400 μg g(-1)) concentrations based on Raman spectral intensity in the region of 2250 cm(-1) was produced.
本文首次报道了对胡萝卜中关键植物营养素（聚乙炔和类胡萝卜素）的快速定量近红外傅里叶变换拉曼分析。通过常规方法对31个胡萝卜品系的溶剂提取物中的这些植物营养素进行了分析，聚乙炔采用气相色谱 - 火焰离子化检测法（GC - FID），类胡萝卜素采用可见分光光度法。类胡萝卜素浓度为0 - 5586μg g(-1)干重（DW）。聚乙炔浓度为74 - 4846μg g(-1) DW，在野生胡萝卜中含量最高。聚乙炔包括镰叶芹醇，含量为6 - 1237μg g(-1) DW；镰叶芹二醇，含量为42 - 3475μg g(-1) DW；以及镰叶芹二醇3 - 乙酸酯，含量为27 - 649μg g(-1) DW。类胡萝卜素的强拉曼谱带与可见分光光度法的结果具有良好的相关性。开发了一种能够从拉曼数据中定量类胡萝卜素的化学计量学模型。基于2250 cm(-1)区域的拉曼光谱强度，建立了一个用于快速区分聚乙炔浓度高和低的胡萝卜的分类模型（检测限 = 1400μg g(-1)）。
