Schülke B, Jacobi K, Schwan K, Schröder A, Tegeler G, Hahn S
Sektion Tierproduktion und Veterinärmedizin der Humboldt-Universität zu Berlin.
Arch Exp Veterinarmed. 1990;44(1):135-42.
Studies were conducted into behaviours of total and extracellular hyaluronidase activities of bull semen under selected incubation phenomena. Particular attention was given to effects generated by media of high and physiological ion strengths (385 mosm and 305 mosm), in vitro capacitation treatment, and effects of various washing techniques as well as incubation temperatures. Total hyaluronidase activity was clearly lowered in washed ejaculate sperms, as compared to original sperm, with extracellular enzyme activity, however, being unchanged. Hyaluronidase activity was also lower in deep-frozen sperm. Increase in extracellular and total hyaluronidase activity was at its highest under the impact of cattle serum albumin and with a defined temperature-time regime (four hours of comparative incubation in a medium of physiological ion strength at 35 degrees C, 37 degrees C, 39 degrees C, 41 degrees C, and 45 degrees C). Release of hyaluronidase was most clearly inducible after pretreatment in medium of physiological ion strength and by four hours of incubation at 39 degrees C. The preliminary conclusion is that the values so far recorded appeared to reflect some superimposition of physiological and degenerative processes which could not be distinguished from each other under the experimental conditions chosen for the studies.
针对选定的孵育现象，对公牛精液中总透明质酸酶活性和细胞外透明质酸酶活性的行为进行了研究。特别关注了高离子强度和生理离子强度（385毫渗量摩尔和305毫渗量摩尔）的培养基、体外获能处理、各种洗涤技术以及孵育温度所产生的影响。与原始精子相比，洗涤后的射精精子中总透明质酸酶活性明显降低，然而细胞外酶活性未发生变化。冷冻精子中的透明质酸酶活性也较低。在牛血清白蛋白的影响下以及在特定的温度 - 时间条件下（在生理离子强度的培养基中于35摄氏度、37摄氏度、39摄氏度、41摄氏度和45摄氏度进行四小时的对比孵育），细胞外和总透明质酸酶活性的增加最为显著。在生理离子强度的培养基中进行预处理并在39摄氏度孵育四小时后，透明质酸酶的释放最易被诱导。初步结论是，迄今为止记录的值似乎反映了生理过程和退化过程的某种叠加，在本研究选择的实验条件下，这两者无法相互区分。
