秋葵（Abelmoschus esculentus L.）提取物的成分分析与免疫调节作用。
Composition analysis and immuno-modulatory effect of okra (Abelmoschus esculentus L.) extract.
机构信息
Department of Food Science, National Pingtung University of Science and Technology, No. 1 Shuehfu Rd., Neipu, Pingtung 91201, Taiwan, ROC.
出版信息
Food Chem. 2012 Oct 15;134(4):1906-11. doi: 10.1016/j.foodchem.2012.03.110. Epub 2012 Apr 3.
The aim of this study was to analyse the composition of okra (Abelmoschus esculentus L.) extract and investigate the effect of A. esculentus L. polysaccharides (AE-PS) on the maturation and function of dendritic cells (DCs) derived from rat bone marrow hematopoietic cells (BMHCs) in vitro. BMHC-derived immature DCs (BMHC-imDCs) were extracted from rats and treated with AE-PS. The hydrolysed okra extract contained 0.6% β-1, 3-D-glucan. AE-PS induced the presence of polymorphic nuclei and elongated protrusion in the BHMC-imDCs, indicating DC activation. Treatment with 100 μg/mL of AE-PS increased the MHC class II and CD80/86 expression levels by 41% and 42%, respectively. Treated cells had reduced endocytosis activity. The secretion of IL-12 and IFN-γ increased significantly by 120% and 75%, respectively, when treated with 100 μg/mL of AE-PS. Moreover, IL-10 production was reduced by 66%. In conclusion, AE-PS exhibits stimulatory effects on rat dendritic cells and promotes the secretion of T(H)1 cytokines.
本研究旨在分析秋葵（Abelmoschus esculentus L.）提取物的组成，并研究 A. esculentus L.多糖（AE-PS）对体外大鼠骨髓造血细胞（BMHC）来源的未成熟树突状细胞（BMHC-imDCs）成熟和功能的影响。从大鼠中提取 BMHC 来源的未成熟 DC（BMHC-imDCs），并用 AE-PS 处理。水解的秋葵提取物含有 0.6%的β-1,3-D-葡聚糖。AE-PS 诱导 BHMC-imDCs 出现多形核和伸长的突起，表明 DC 激活。用 100μg/mL 的 AE-PS 处理后，MHC Ⅱ类和 CD80/86 的表达水平分别增加了 41%和 42%。处理后的细胞内吞活性降低。用 100μg/mL 的 AE-PS 处理后，IL-12 和 IFN-γ 的分泌分别显著增加了 120%和 75%，而 IL-10 的产生则降低了 66%。总之，AE-PS 对大鼠树突状细胞具有刺激作用，并促进 T(H)1 细胞因子的分泌。
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