Nievelstein P F, de Groot P G, D'Alessio P, Heijnen H F, Orlando E, Sixma J J
Department of Haematology, University Hospital Utrecht, The Netherlands.
Arteriosclerosis. 1990 May-Jun;10(3):462-9. doi: 10.1161/01.atv.10.3.462.
Platelet deposition on cultured fibroblasts and on their extracellular matrix (FBM) was investigated in a flow system with citrated blood and was compared with platelet deposition on cultured endothelial cells, smooth muscle cells, and their extracellular matrices. Platelet deposition was present at all surfaces except on intact endothelial cells. Deposition on FBM consisted of contact platelets, spread platelets, and a few small aggregates. On intact fibroblasts cells, the surface coverage was lower, and platelets formed aggregates. Factors involved in primary hemostasis, particularly the wall shear rate, von Willebrand factor (vWF), and fibronectin, were investigated on FBM. The reactivity of FBM was determined by the passage number of the cultured cells. The vWF was involved in platelet adhesion on FBM at only the high shear rate (greater than 800 s-1). Platelet deposition was independent of plasma fibronectin at all shear rates tested. Matrix-associated fibronectin was involved in adhesion at low and high wall shear rates. We conclude that FBM can be used as a platelet adhesive surface especially to study the contribution of exogenous vWF to platelet adhesion because FBM does not contain vWF.
在一个使用枸橼酸盐血液的流动系统中，研究了血小板在培养的成纤维细胞及其细胞外基质（FBM）上的沉积情况，并将其与血小板在培养的内皮细胞、平滑肌细胞及其细胞外基质上的沉积进行了比较。除了完整的内皮细胞表面外，在所有表面均出现了血小板沉积。FBM上的沉积包括接触血小板、铺展血小板和一些小聚集体。在完整的成纤维细胞上，表面覆盖率较低，血小板形成聚集体。在FBM上研究了参与初级止血的因素，尤其是壁剪切率、血管性血友病因子（vWF）和纤连蛋白。FBM的反应性由培养细胞的传代次数决定。vWF仅在高剪切率（大于800 s-1）时参与FBM上的血小板黏附。在所有测试的剪切率下，血小板沉积均与血浆纤连蛋白无关。基质相关纤连蛋白在低壁剪切率和高壁剪切率下均参与黏附。我们得出结论，FBM可作为血小板黏附表面，特别是用于研究外源性vWF对血小板黏附的贡献，因为FBM不含vWF。
