Sinclair A
Department of Wool and Animal Science, University of New South Wales, Kensington.
Aust Vet J. 1990 Feb;67(2):59-62. doi: 10.1111/j.1751-0813.1990.tb07696.x.
Skin biopsies from two Merino sheep heavily infested with Psorergates ovis were immersed in liquid nitrogen and cut into vertical frozen sections stained with lipophilic Sudan IV or Oil Red O and haematoxylin. A survey of the lateral distribution of mite sections showed a majority (ca 80%) were in or within 0.2 mm of the follicle mouth. A survey of vertical distribution showed no mite penetration deeper than inner stratum corneum where 57% of mite sections were seen; 30% were within outer stratum corneum or scurf; 13% were on the outer surface and less than 1% were detached. Lipid was the only material seen within stained mites at a location considered to be gut. This was supported by dosing sheep with quinacrine, taking biopsies at Day 6 and Day 14 and examining frozen sections under blue light. Fluorescent lipid was seen at a location considered to be mite gut. From these results it was recommended that acaricide treatments against P. ovis be lipophilic and administered transepidermally because less than 15% of mites were at a superficial location likely to be reached by topical application.
从两只严重感染绵羊痒螨的美利奴羊身上采集皮肤活检样本，将其浸入液氮中，切成垂直冰冻切片，用亲脂性苏丹IV或油红O以及苏木精染色。对螨切片的横向分布进行调查发现，大多数（约80%）位于毛囊口或距毛囊口0.2毫米范围内。对垂直分布的调查显示，没有螨穿透超过角质层内层，57%的螨切片可见于此；30%位于角质层外层或皮屑内；13%位于外表面，不到1%处于游离状态。在被认为是肠道的位置，脂质是在染色螨体内见到的唯一物质。给绵羊服用奎纳克林，在第6天和第14天采集活检样本并在蓝光下检查冰冻切片，这支持了上述结论。在被认为是螨肠道的位置可见荧光脂质。根据这些结果，建议用于防治绵羊痒螨的杀螨剂应为亲脂性且经皮给药，因为不到15%的螨处于可能通过局部应用到达的浅表位置。
