[黑寡妇蜘蛛神经毒素受体的研究。I. 来自牛脑的膜结合型和可溶型受体的特性]
[Study of the receptor for black widow spider neurotoxin. I. Characteristics of membrane-bound and solubilized receptors from the bovine brain].
作者信息
Petrenko A G, Shamotienko O G, Surkova I N, Kovalenko V A, Grishin E V
出版信息
Bioorg Khim. 1990 Feb;16(2):149-57.
Iodine-125 labelled alpha-latrotoxin from the venom of Central Asia black widow spider Latrodectus mactans tredecimguttatus binds specifically to the bovine brain membrane receptor producing a stable slowly dissociating complex with Kd = 1.6 x 10(-10) M and Bmax = 0.5 pmol/mg protein. Treatment of the complex with alkaline high-salt buffer induces reversible dissociation of the bound toxin. The antitoxin polyclonal antibody does not increase the dissociation rate of the bound toxin. Wheat germ lectin as well as concanavalin A inhibit the toxin binding to the membrane receptor. The receptor is solubilized with ionic and non-ionic detergents, and methods of latrotoxin binding assay are developed. The solubilized receptor is shown to retain high affinity to toxin, its binding activity being stable but critically dependent on the presence of calcium ions. Chromatographic properties of the receptor suggest its glycoprotein nature.
来自中亚黑寡妇蜘蛛（间斑寇蛛）毒液的碘 - 125标记的α - 拉毒素特异性结合牛脑膜受体，形成稳定的缓慢解离复合物，解离常数Kd = 1.6×10⁻¹⁰ M，最大结合量Bmax = 0.5 pmol/mg蛋白质。用碱性高盐缓冲液处理该复合物可诱导结合毒素的可逆解离。抗毒素多克隆抗体不会增加结合毒素的解离速率。麦胚凝集素以及伴刀豆球蛋白A可抑制毒素与膜受体的结合。该受体可用离子型和非离子型去污剂溶解，并开发了拉毒素结合测定方法。结果表明，溶解的受体对毒素仍保持高亲和力，其结合活性稳定，但严重依赖钙离子的存在。受体的色谱特性表明其为糖蛋白性质。
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