Trikash I O, Lishko V K, Grebinozhko E I, Mishchenko N I
Ukr Biokhim Zh (1978). 1986 Nov-Dec;58(6):50-7.
Interaction of latrotoxin with phospholipid vesicles and bilayer lipid membranes is shown to proceed differently. Latrotoxin when interacting with liposomes is sorbed on the membrane surface forming no ionic channels in this case. Only latrotoxin fragments obtained due to the toxin hydrolysis by pronase or trypsin are able to form channels. These fragments being inserted into liposomes are coupled strongly with the membrane and are not subjected to the further splitting by proteinases. The electrophoretic spectrum of peptides bound with liposomes is presented by protein components with a molecular weight of 116, 100, 92, 67, 52 and 45 kDa, while zone typical of latrotoxin is absent in this spectrum. The method of small-angle X-rays scattering has shown that tryptic fragments of latrotoxin penetrate into the phospholipid bilayer of liposomes.
研究表明，Latrotoxin与磷脂囊泡和双层脂质膜的相互作用方式不同。Latrotoxin与脂质体相互作用时，吸附在膜表面，此时不形成离子通道。只有经链霉蛋白酶或胰蛋白酶水解毒素产生的Latrotoxin片段才能形成通道。这些片段插入脂质体后与膜紧密结合，不再被蛋白酶进一步裂解。与脂质体结合的肽的电泳图谱由分子量为116、100、92、67、52和45 kDa的蛋白质成分组成，而该图谱中没有Latrotoxin的典型区域。小角X射线散射法表明，Latrotoxin的胰蛋白酶片段可穿透脂质体的磷脂双层。
