Uehara Shuichiro, Oue Takaharu, Kawatsu Miyoko, Nara Keigo, Fukuzawa Masahiro
Department of Pediatric Surgery, Osaka University, Osaka, Japan.
Eur J Pediatr Surg. 2013 Oct;23(5):400-4. doi: 10.1055/s-0033-1333637. Epub 2013 Feb 26.
Survivin, an inhibitor of apoptosis, has been reported to be associated with a worse prognosis in some malignancies. However, its expression in hepatoblastoma (HB) remains to be elucidated. We assessed the survivin expression in HB specimens collected before and after chemotherapy to elucidate the impact of survivin on the outcome of HB therapy.
HB specimens were collected before and after 2 to 4 cycles of cisplatin-based chemotherapy from 16 patients. The survivin expression level was assessed by immunohistochemical staining and real-time polymerase chain reaction.
Out of 16, 12 HB sections collected before chemotherapy were positive for survivin as determined by immunohistochemical staining. The intensity of survivin expression was found to significantly increase after chemotherapy. Surprisingly, all of the HB specimens obtained after chemotherapy were positive for survivin. The expression of survivin messenger ribonucleic acid from a human HB cell line, Huh-6 was significantly higher when the cells were cultured with cis-diamminedichloroplatinum(II) than when the cells were cultured without the drug.
Our results indicate that most of the primary HB tissue specimens express survivin, and its expression increased after chemotherapy, thus suggesting that survivin may concern with the survival of tumor cells, therefore be a candidate for the target of the treatment of HB.
凋亡抑制蛋白Survivin在一些恶性肿瘤中与预后较差相关。然而，其在肝母细胞瘤（HB）中的表达仍有待阐明。我们评估了化疗前后收集的HB标本中Survivin的表达，以阐明Survivin对HB治疗结果的影响。
从16例患者中收集基于顺铂的化疗2至4个周期前后的HB标本。通过免疫组织化学染色和实时聚合酶链反应评估Survivin的表达水平。
免疫组织化学染色显示，16例中化疗前收集的12个HB切片Survivin呈阳性。发现化疗后Survivin表达强度显著增加。令人惊讶的是，化疗后获得的所有HB标本Survivin均呈阳性。当人HB细胞系Huh-6与顺二氯二氨铂（II）一起培养时，其Survivin信使核糖核酸的表达明显高于无药物培养时。
我们的结果表明，大多数原发性HB组织标本表达Survivin，且化疗后其表达增加，这表明Survivin可能与肿瘤细胞的存活有关，因此可能是HB治疗靶点的候选者。
