Domingo N, Botta D, Martigne-Cros M, Lechêne de la Porte P, Pak-Leung P, Hauton J, Lafont H
Unité 130 de l'INSERM, Marseille, France.
Biochim Biophys Acta. 1990 May 22;1044(2):243-8. doi: 10.1016/0005-2760(90)90309-l.
Bile lipids are thought to be secreted in a lipoprotein complex in which they are associated with cholesterol and a protein called the anionic polypeptidic fraction (APF). APF is present in both bile and serum HDL. The association of APF with both bile and lipoprotein strongly suggests that hepatocytes may be responsible for the synthesis and secretion of this protein. In the present work we attempted to verify this by studying the incorporation of [14C]leucine into APF in isolated rat hepatocytes and by immunolocalization in cell cultures. Results obtained showed that synthesis of APF by cells follows the same kinetic pattern as albumin and that it was the third most abundant protein in the bile secretion. Immunolocalization confirmed that APF is synthesized in the endoplasmic reticulum of hepatocytes. This protein which appears to be rapidly secreted could be of great value for the specific detection of the lipids destined for bile secretion.
胆汁脂质被认为是以脂蛋白复合物的形式分泌的，在该复合物中，它们与胆固醇以及一种称为阴离子多肽部分（APF）的蛋白质相关联。APF存在于胆汁和血清高密度脂蛋白（HDL）中。APF与胆汁和脂蛋白的关联强烈表明，肝细胞可能负责这种蛋白质的合成和分泌。在本研究中，我们试图通过研究[14C]亮氨酸在分离的大鼠肝细胞中掺入APF的情况以及通过细胞培养中的免疫定位来验证这一点。获得的结果表明，细胞合成APF的动力学模式与白蛋白相同，并且它是胆汁分泌中第三丰富的蛋白质。免疫定位证实APF是在肝细胞的内质网中合成的。这种似乎能快速分泌的蛋白质对于特异性检测 destined for胆汁分泌的脂质可能具有重要价值。
原文中“destined for”表述有误，可能是“destined to”，但按照要求未做修改，翻译后的内容中保留了原文错误表述。 正确的句子应该是“这种似乎能快速分泌的蛋白质对于特异性检测 注定要进入胆汁分泌的脂质可能具有重要价值。” 。
