Laboratory of Applied Entomology and Zoology, Faculty of Agriculture, Ibaraki University, Ami, Ibaraki 300-0393, Japan.
J Econ Entomol. 2013 Feb;106(1):463-72. doi: 10.1603/ec12328.
Spider mites are difficult to identify because they are very small and have a limited number of diagnostic characters. Most species of the spider mite genus Tetranychus in Japan are morphologically similar, differing only in the diameter of the aedeagal knob in males. Because this genus contains many important pests, the unambiguous identification of species is crucial for effective pest management and quarantine procedures. DNA-based methods could complement the morphological methods. We examined whether Tetranychus species in Japan could be identified by DNA sequences using the internal transcribed spacer region of nuclear ribosomal DNA and the cytochrome c oxidase subunit I gene of mitochondrial DNA. We determined sequences of the 13 known Tetranychus species in Japan. We could identify 10 of the 13 species in the internal transcribed spacer tree. In the cytochrome c oxidase subunit I tree, we could identify all 13 known Tetranychus species in Japan. Although Tetranychus kanzawai Kishida and T. parakanzawai Ehara were identified by DNA sequences, they were clearly separated into two monophyletic clades each, indicating that a cryptic species existed in each species.
叶螨很难识别，因为它们非常小，并且具有有限数量的诊断特征。在日本，大多数叶螨属 Tetranychus 的物种在形态上相似，仅在雄性的精荚旋钮直径上有所不同。由于该属包含许多重要的害虫，因此物种的明确鉴定对于有效的害虫管理和检疫程序至关重要。基于 DNA 的方法可以补充形态学方法。我们检查了日本的 Tetranychus 物种是否可以通过核核糖体 DNA 的内部转录间隔区和线粒体 DNA 的细胞色素 c 氧化酶亚基 I 基因的 DNA 序列来识别。我们确定了日本已知的 13 种 Tetranychus 物种的序列。我们可以在内部转录间隔树中识别出 10 种 13 种已知的 Tetranychus 物种。在线粒体 DNA 的细胞色素 c 氧化酶亚基 I 树中，我们可以识别出日本的所有 13 种已知的 Tetranychus 物种。尽管 DNA 序列鉴定出了 Kishida 的 Tetranychus kanzawai 和 Ehara 的 T. parakanzawai，但它们分别清楚地分为两个单系群，表明每个物种中都存在隐种。
