从粪便堆肥中鉴定产嗜热水解酶的耐热细菌菌株。
Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost.
机构信息
Département de Chimie-Biologie, Université du Québec à Trois-Rivières, 3351 Boul. Des Forges, C.P. 500, Trois-Rivières (Québec), G9A 5H7 Canada.
出版信息
Indian J Microbiol. 2012 Mar;52(1):41-7. doi: 10.1007/s12088-011-0156-8. Epub 2011 Feb 3.
Abstract
Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60-65°C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, α-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications.
摘要
从粪肥堆肥中分离出了 10 株嗜热细菌菌株。基于 16S rRNA 基因的系统发育分析和生化特征鉴定，确定了属于四个属的四个不同种：嗜热地芽孢杆菌、史密斯芽孢杆菌、苏旺尼亚芽孢杆菌和嗜热好氧菌。16S-ITS-23S rRNA 区的 PCR-RFLP 图谱允许我们区分 G. thermodenitrificans 分离株中的两个亚群。对耐热水解活性（60-65°C）进行了筛选。在 G. thermodenitrificans、A. thermoaerophilus 和 B. smithii 中检测到耐热脂肪酶活性。在 G. thermodenitrificans 组中还观察到耐热蛋白酶、α-淀粉酶和木聚糖酶活性。这些物种代表了工业应用中具有潜在新型耐热酶的来源。
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