Mothersill C, Seymour C B, Bonnar J
Saint Luke's Hospital, Rathgar, Dublin, Ireland.
Gynecol Oncol. 1990 May;37(2):210-8. doi: 10.1016/0090-8258(90)90335-i.
A technique is presented which allows the response of human gynecological tissue to radiation and cytotoxic drugs to be assessed using a tissue culture explant system. The technique is simple to use and gives results in line with those obtained for human tissues by more complex culture methods. Data are presented showing how the explant technique developed by the group for other tissues can be adapted to yield acceptable results for normal tissue response to radiation. The potential of the technique for use in predictive testing of individual tumor response is then assessed in five cases of gynecological malignancy. It is clear that variations in sensitivity to different radio- and chemotherapy agents and combinations can be detected. The results obtained require clinical validation and it is hoped that this will come over the next few years from evaluation of patient response to treatment using individually optimized, rather empirical therapy.
本文介绍了一种技术，该技术可使用组织培养外植体系统评估人类妇科组织对辐射和细胞毒性药物的反应。该技术使用简便，所得结果与通过更复杂培养方法获得的人类组织结果一致。文中给出的数据表明，该团队为其他组织开发的外植体技术如何经过调整，以得出正常组织对辐射反应的可接受结果。随后，在5例妇科恶性肿瘤病例中评估了该技术用于预测个体肿瘤反应的潜力。显然，可以检测到对不同放疗和化疗药物及其组合的敏感性差异。所获得的结果需要临床验证，希望在未来几年通过评估患者对使用个体化优化而非经验性治疗的反应来实现这一点。
