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关于细菌基因组的突变拓扑结构。

On the mutational topology of the bacterial genome.

机构信息

Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.

出版信息

G3 (Bethesda). 2013 Mar;3(3):399-407. doi: 10.1534/g3.112.005355. Epub 2013 Mar 1.

Abstract

By sequencing the genomes of 34 mutation accumulation lines of a mismatch-repair defective strain of Escherichia coli that had undergone a total of 12,750 generations, we identified 1625 spontaneous base-pair substitutions spread across the E. coli genome. These mutations are not distributed at random but, instead, fall into a wave-like spatial pattern that is repeated almost exactly in mirror image in the two separately replicated halves of the bacterial chromosome. The pattern is correlated to genomic features, with mutation densities greatest in regions predicted to have high superhelicity. Superimposed upon this pattern are regional hotspots, some of which are located where replication forks may collide or be blocked. These results suggest that, as they traverse the chromosome, the two replication forks encounter parallel structural features that change the fidelity of DNA replication.

摘要

通过对 34 条经历了总共 12750 代的错配修复缺陷型大肠杆菌突变积累系的基因组进行测序,我们在大肠杆菌基因组中鉴定出了 1625 个自发的碱基对替换。这些突变并非随机分布,而是呈现出一种类似波浪的空间模式,在细菌染色体的两个独立复制半体中几乎可以完全镜像重复。该模式与基因组特征相关联,突变密度在预测具有高超螺旋的区域最大。在此模式之上叠加了区域热点,其中一些位于复制叉可能碰撞或受阻的位置。这些结果表明,当两个复制叉穿过染色体时,它们会遇到改变 DNA 复制保真度的平行结构特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/008a/3583449/7b31d00f3b8c/399f1.jpg

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