Harnly James M, Luthria Devanand, Chen Pei
U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Food Composition and Methods Development Laboratory, Building-161, BARC-East, 10300 Baltimore Ave, Beltsville, MD 20705, USA.
J AOAC Int. 2012 Nov-Dec;95(6):1579-87. doi: 10.5740/jaoacint.12-096.
The fingerprints of 18 commercially available Ginkgo biloba supplements, 12 samples of raw G. biloba leaves, and three G. biloba standard reference materials from the National Institute of Standards and Technology were acquired directly (no chromatography) by UV spectrometry and after separation using HPLC with a diode array detector. The fingerprints consisted of the UV spectral images, the chromatographic images, and the areas of the 21 most prominent chromatographic peaks. Data were analyzed by principal component analysis and one-class soft independent modeling of class analogy (SIMCA). It was determined that three of the commercial products were adulterated with rutin, four with quercetin, and one with an unidentified flavonol glycoside. One-class SIMCA of the authentic products allowed the adulterated products to be easily distinguished using Q-residuals. Authentic supplements and raw leaf materials were easily distinguished. The finely powdered samples were also analyzed by near-IR (NIR) spectrometry. The authentic and adulterated products could not be distinguished by NIR spectrometry because of the excipients.
通过紫外光谱法直接（无需色谱法）以及使用配备二极管阵列检测器的高效液相色谱法分离后，获取了18种市售银杏补充剂、12份银杏叶原料样本以及三种来自美国国家标准与技术研究院的银杏标准参考物质的指纹图谱。这些指纹图谱包括紫外光谱图像、色谱图像以及21个最显著色谱峰的面积。数据通过主成分分析和一类软独立类比建模（SIMCA）进行分析。结果确定，三种商业产品掺有芦丁，四种掺有槲皮素，一种掺有未鉴定的黄酮醇苷。正品的一类SIMCA分析使得掺假产品能够通过Q残差轻松区分。正品补充剂和原料叶很容易区分。细粉状样品也通过近红外（NIR）光谱法进行了分析。由于辅料的原因，近红外光谱法无法区分正品和掺假产品。
