Pate J L, De Jong D M
Department of Bacteriology, University of Wisconsin, Madison 53706.
J Bacteriol. 1990 Jun;172(6):3117-24. doi: 10.1128/jb.172.6.3117-3124.1990.
Nonmotile mutants of the gliding bacterium Cytophaga johnsonae were examined to identify proteins that might be involved in gliding motility. Wild-type and mutant cell proteins were solubilized and fractionated by using Triton X-114, and the proteins that partitioned into the aqueous phase or the detergent phase were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for proteins that differed between wild-type and mutant cells. Seventeen proteins, ranging in size from 16 to 150 kilodaltons, were implicated by this technique as having some relationship to gliding and were designated Gld-1 through Gld-17. All Gld proteins behaved as integral membrane proteins, partitioning into the detergent phase. All 56 mutants examined exhibited changes in 1 or more of the Gld proteins, with the number of proteins altered in any mutant varying from 1 to 11. Several lines of evidence suggested that proteins Gld-12 through Gld-15 are glycoproteins. Analysis of banding patterns of detergent-fraction proteins of motile revertants supported the idea that the Gld proteins have a role in gliding motility.
对滑行细菌约翰逊噬纤维菌的非运动突变体进行了检测，以鉴定可能参与滑行运动的蛋白质。野生型和突变体细胞蛋白通过使用Triton X-114进行溶解和分级分离，然后通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析分配到水相或去污剂相中的蛋白质，以找出野生型和突变体细胞之间存在差异的蛋白质。通过该技术鉴定出17种蛋白质，大小从16至150千道尔顿不等，它们与滑行运动存在某种关联，并被命名为Gld-1至Gld-17。所有Gld蛋白均表现为整合膜蛋白，分配到去污剂相中。所检测的56个突变体均在1种或更多种Gld蛋白上出现变化，任何一个突变体中发生改变的蛋白数量从1至11不等。几条证据表明，Gld-12至Gld-15蛋白是糖蛋白。对运动回复体去污剂分级分离蛋白的条带模式分析支持了Gld蛋白在滑行运动中起作用的观点。
