采用带火焰离子化检测器的气相色谱法测定橄榄油混合物中蜡含量的变化。

Changes of the wax contents in mixtures of olive oils as determined by gas chromatography with a flame ionization detector.

作者信息

Hodaifa Gassan, MartínezNieto Leopoldo, Lozano Juan L, Sánchez Sebastián

机构信息

University of Pablo de Olavide, Molecular Biology and Biochemical Engineering Department, 41013 Seville, Spain.

出版信息

J AOAC Int. 2012 Nov-Dec;95(6):1720-4. doi: 10.5740/jaoacint.12-011.

DOI:10.5740/jaoacint.12-011

PMID:23451389

Abstract

Mixing of refined olive-pomace oil with virgin olive oil is a fraud that has been tried often. Normally, the tests that detected the fraud were determinations of wax esters, erythrodiol+uvaol, and stigmastadienes contents. The most common is the determination of wax esters content (extra virgin olive oil is very poor in wax esters, usually less than 100 mg/kg). In this work, the variations of individual wax esters (C40, C42, C44, and C46), with different degrees of unsaturation content, and total wax esters were studied when extra virgin olive oil and refined pomace-olive oil were mixed. The following mixtures were prepared: extra virgin olive oil plus 1, 2, 4, 6, 8, 10, 12, 15, 18, 25, 35, 45, 50, and 80% of refined olive-pomace oil. In all cases, individual and total wax ester content variation was linear with increasing percentage of refined olive-pomace oil in the mixture. The variation of the total wax esters content can be adjusted according to the equation: Total wax esters, mg/kg = 14.3 x (% refined olive-pomace oil) + 83.9.

摘要

将精炼橄榄果渣油与初榨橄榄油混合是一种屡试不爽的欺诈行为。通常，检测此类欺诈行为的测试是测定蜡酯、赤藓醇+乌索醇以及豆甾二烯的含量。最常见的是测定蜡酯含量（特级初榨橄榄油中的蜡酯含量极低，通常低于100毫克/千克）。在本研究中，当特级初榨橄榄油与精炼橄榄果渣油混合时，研究了不同不饱和度的单个蜡酯（C40、C42、C44和C46）以及总蜡酯的变化情况。制备了以下混合物：特级初榨橄榄油分别添加1%、2%、4%、6%、8%、10%、12%、15%、18%、25%、35%、45%、50%和80%的精炼橄榄果渣油。在所有情况下，单个蜡酯和总蜡酯含量的变化与混合物中精炼橄榄果渣油百分比的增加呈线性关系。总蜡酯含量的变化可根据以下方程式进行调整：总蜡酯，毫克/千克 = 14.3×（精炼橄榄果渣油百分比）+ 83.9。