Kerwin J L, Dritz D A, Washino R K
Department of Entomology, University of California, Davis 95616.
J Econ Entomol. 1990 Apr;83(2):374-6. doi: 10.1093/jee/83.2.374.
Lagenidium giganteum Couch, a fungal parasite of mosquito larvae, is considered a promising agent for operational control. Quantitative reisolation of the fungus from rodent blood and tissue is easily accomplished because the cell walls are much more persistent than those of mammalian cells. The mycelium forms discrete, septate cells very early in its development, in marked contrast to filamentous fungi. The fungus is rapidly cleared from mice following intraperitoneal injection of large quantities of mycelium and oospores. More than 95% of viable cells are cleared within 24 h. Mortality following intravenous injection of mycelium in tail veins of mice is caused solely by embolism of heart and lung tissue. Recently revised U.S. Environmental Protection Agency protocols for testing of microbial pest control agents may have to be altered further to accommodate physically large microbial agents such as L. giganteum.
巨大拉格孢菌（Lagenidium giganteum Couch）是一种蚊子幼虫的真菌寄生虫，被认为是一种有前景的用于实际防治的制剂。由于其细胞壁比哺乳动物细胞的细胞壁更具持久性，所以从啮齿动物血液和组织中对该真菌进行定量再分离很容易实现。该真菌的菌丝体在其发育早期就形成离散的、有隔膜的细胞，这与丝状真菌形成鲜明对比。在腹腔注射大量菌丝体和卵孢子后，该真菌能迅速从小鼠体内清除。超过95%的活细胞在24小时内被清除。在小鼠尾静脉注射菌丝体后的死亡率完全是由心脏和肺组织的栓塞引起的。美国环境保护局最近修订的微生物害虫防治剂测试方案可能需要进一步修改，以适应像巨大拉格孢菌这样体积较大的微生物制剂。
