Parkening T A
Department of Anatomy, University of Texas Medical Branch, Galveston 77550.
J Exp Zool. 1990 Jun;254(3):305-12. doi: 10.1002/jez.1402540309.
Siberian hamsters were superovulated and various media were tested in an effort to fertilize the recovered oocytes in vitro. The highest percentage of fertilized ova was achieved by using a modified Tyrode's medium, designated MT (Bavister, J. Reprod. Fertil., 18:544-545, '69), previously formulated to fertilize Syrian hamster ova in vitro. Spermatozoa incubated in this medium in a concentrated state overnight (14 hr) and then diluted (1 hr) fertilized 39% of the ova. Similar results (40%) were obtained with this medium by adding 20% human follicular fluid to fresh concentrated sperm for 30 min and then diluting the sperm for 2-3 hr prior to the addition of ova. Ova fertilized in vitro cleaved to the two-cell stage but failed to develop any further in culture. Two-cell embryos recovered from mated hamsters and cultured did not undergo additional cleavage. Four-cell embryos collected from mated females and cultured cleaved to the six- to eight-cell stage and stopped. Techniques and media used for fertilizing large numbers of Syrian and Chinese hamster ova in vitro will have to be modified to achieve the same degree of success in the Siberian hamster.
对西伯利亚仓鼠进行超数排卵，并测试了各种培养基，试图使回收的卵母细胞在体外受精。通过使用一种改良的台氏液培养基（称为MT，Bavister，《生殖与受精杂志》，18:544 - 545，'69），获得了最高的受精卵百分比，该培养基先前被配制用于使叙利亚仓鼠卵母细胞在体外受精。精子在这种培养基中以浓缩状态孵育过夜（14小时），然后稀释（1小时），使39%的卵受精。通过在新鲜浓缩精子中加入20%人卵泡液30分钟，然后在加入卵之前将精子稀释2 - 3小时，使用这种培养基也获得了类似的结果（40%）。体外受精的卵分裂到二细胞阶段，但在培养中未能进一步发育。从交配的仓鼠中回收并培养的二细胞胚胎没有进行额外的分裂。从交配的雌性仓鼠中收集并培养的四细胞胚胎分裂到六至八细胞阶段后停止。用于使大量叙利亚和中国仓鼠卵母细胞在体外受精的技术和培养基必须进行改进，以便在西伯利亚仓鼠中取得同样程度的成功。
