Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA.
J Invertebr Pathol. 2013 May;113(1):82-5. doi: 10.1016/j.jip.2013.02.004. Epub 2013 Feb 27.
White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp. The major targets of WSSV infection are tissues of ectodermal and mesodermal embryonic origin, predominantly the cuticular epithelium and subcuticular connective tissues. Recently, we discovered a WSSV variant in Penaeus indicus that heavily infects the subcuticular connective tissue, with very slight indications in the cuticular epithelium. The variant was also unusual in that WSSV accumulations were found in the interstitial spaces of both the subcuticular connective tissue and the lymphoid organ. This WSSV variant was confirmed through immunohistochemistry with an anti-WSSV VP28 monoclonal antibody, and also by in situ hybridization with a VP28 DNA probe. By in situ hybridization, shrimp with variant and typical histology were shown a deletion in ORF94, which is characteristic of a new type of WSSV found in Saudi Arabia; apparently, the loss of this ORF is not associated with the variant's reduced capability of infecting the cuticular epithelium cells.
白斑综合征病毒（WSSV）对虾类具有高度致病性。WSSV 的主要感染靶标是外胚层和中胚层胚胎起源的组织，主要是表皮和皮下结缔组织。最近，我们在印度对虾中发现了一种 WSSV 变体，它严重感染皮下结缔组织，表皮上皮组织只有轻微的感染迹象。该变体还不寻常，因为在皮下结缔组织和淋巴器官的间质空间中都发现了 WSSV 聚集。通过用抗 WSSV VP28 单克隆抗体进行免疫组织化学和用 VP28 DNA 探针进行原位杂交，证实了这种 WSSV 变体的存在。通过原位杂交，显示具有变体和典型组织学的虾缺失 ORF94，这是在沙特阿拉伯发现的新型 WSSV 的特征；显然，这种 ORF 的缺失与该变体降低感染表皮上皮细胞的能力无关。
