评估alkB末端限制性片段和序列类型在不同细菌分类群中的分配情况。
Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.
作者信息
Giebler Julia, Wick Lukas Y, Schloter Michael, Harms Hauke, Chatzinotas Antonis
机构信息
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research, Leipzig, Germany.
出版信息
Appl Environ Microbiol. 2013 May;79(9):3129-32. doi: 10.1128/AEM.04028-12. Epub 2013 Mar 1.
Abstract
Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.
摘要
序列分析和末端限制性片段长度多态性（T-RFLP）分析显示，土壤细菌分离株中每个细胞存在多个alkB基因拷贝，并且不同系统发育组之间具有明显较高的遗传流动性。通过alkB末端限制性片段（T-RFs）和序列来鉴定烷烃降解菌存在很大偏差，因为基于16S rRNA和alkB基因序列构建的系统发育树高度不一致。
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