Department of Gastroenterology and Central Laboratory, The Central Hospital of Wuhan, Sheng Li Street 26, Wuhan, 430014, Hubei Province, People's Republic of China.
Int J Colorectal Dis. 2013 Oct;28(10):1351-8. doi: 10.1007/s00384-013-1671-3. Epub 2013 Mar 3.
Our aims were to evaluate protein tyrosine phosphatase nonreceptor type 22 (PTPN22) gene polymorphisms in ulcerative colitis (UC) and explore PTPN22 mRNA levels in colonic biopsies of UC patients in central China.
A total of 165 Chinese UC patients and 300 healthy controls were enrolled in this study. PTPN22 -1123G/C, +1858C/T, and +788G/A polymorphisms were genotyped by PCR-restriction fragment length polymorphism method. PTPN22 mRNA expressions in colonic biopsies and serum C-reactive protein (CRP) levels were determined by quantitative PCR and immunonephelometry, respectively.
The frequency of C carrier was higher in UC patients than in healthy controls (66.7 vs. 53.3%, P = 0.005, odds ratios = 1.75, 95% CI 1.18-2.60) and associated with extensive colitis (P = 0.029). PTPN22 mRNA levels were elevated in UC patients than in healthy controls (P < 0.001). Among UC patients, PTPN22 mRNA expression levels were higher in biopsies of inflamed colonic tissue compared with noninflamed tissue (P < 0.001) and were correlated with CRP levels (r = 0.578, P < 0.001). PTPN22 mRNA expression levels were elevated in extensive colitis compared to proctitis (P = 0.008) and to left-sided colitis (P = 0.029) and were higher in moderate and severe disease than in mild disease (P = 0.005).
Our study showed the potential association between PTPN22 -1123G/C polymorphism and UC in central China. PTPN22 mRNA levels were highly expressed in UC, especially in active disease, and were correlated with CRP levels, disease location, and disease severity in UC patients.
本研究旨在评估蛋白酪氨酸磷酸酶非受体型 22（PTPN22）基因多态性在溃疡性结肠炎（UC）中的作用，并探讨中国中部地区 UC 患者结肠活检组织中 PTPN22 mRNA 的水平。
本研究纳入了 165 例中国 UC 患者和 300 名健康对照者。采用 PCR-限制性片段长度多态性方法检测 PTPN22-1123G/C、+1858C/T 和+788G/A 多态性。采用实时定量 PCR 和免疫比浊法分别检测结肠活检组织和血清 C 反应蛋白（CRP）水平。
UC 患者中 C 等位基因的频率高于健康对照者（66.7%比 53.3%，P=0.005，比值比=1.75，95%可信区间 1.18-2.60），且与广泛性结肠炎相关（P=0.029）。UC 患者的 PTPN22 mRNA 水平高于健康对照者（P<0.001）。在 UC 患者中，炎症性结肠组织的 PTPN22 mRNA 表达水平高于非炎症性组织（P<0.001），且与 CRP 水平相关（r=0.578，P<0.001）。广泛性结肠炎患者的 PTPN22 mRNA 表达水平高于直肠炎患者（P=0.008）和左半结肠炎患者（P=0.029），且中重度疾病患者高于轻度疾病患者（P=0.005）。
本研究显示 PTPN22-1123G/C 多态性与中国中部地区 UC 之间存在潜在的相关性。PTPN22 mRNA 在 UC 中高度表达，尤其是在活动期疾病中，与 CRP 水平、疾病部位和疾病严重程度相关。
