Swartjes Maarten, Niesters Marieke, Dahan Albert
Department of Anesthesiology, Leiden University Medical Center, Leiden, The Netherlands.
Methods Mol Biol. 2013;982:187-95. doi: 10.1007/978-1-62703-308-4_12.
Neuropathic pain following nerve injury is a chronic disease characterized by allodynia and hyperalgesia of either mechanical or thermal origin. The mechanism underlying this disease is poorly understood leading to pharmacologic and physiotherapeutic control that is often insufficient. In this chapter, we describe a method to induce nerve injury in rats to create a robust animal model for studying neuropathic pain. Additionally we describe a method to follow up on animals in the process of testing treatments for efficacy in alleviating allodynia by testing for both mechanical and thermal allodynia with reproducible results.
神经损伤后出现的神经性疼痛是一种慢性疾病，其特征为源于机械性或热性刺激的痛觉过敏和异常性疼痛。该疾病的潜在机制尚未完全明确，导致药物治疗和物理治疗的效果往往欠佳。在本章中，我们描述了一种在大鼠中诱导神经损伤的方法，以创建一个用于研究神经性疼痛的可靠动物模型。此外，我们还描述了一种在测试治疗方法缓解异常性疼痛疗效的过程中对动物进行随访的方法，即通过检测机械性和热性异常性疼痛来获得可重复的结果。
